Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells

Montes, Rosa; Romero, Tamara; Cabrera, Sonia; Ayllon, Veronica; Lopez-Escamez, Jose Antonio; Ramos-Mejia, Veronica; Real, Pedro Jose

doi:10.1016/j.scr.2015.10.009

2015 MontesR_StemCellResearch.pdf (1.237Mb)

Identificadores

URI: https://hdl.handle.net/10481/105929

DOI: 10.1016/j.scr.2015.10.009

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Editorial

Elsevier

Fecha

2015-11

Referencia bibliográfica

R. Montes et al. / Stem Cell Research 15 (2015) 614–617. https://doi.org/10.1016/j.scr.2015.10.009

Patrocinador

Ministry of Economy and Competitiveness (JCI_2012_12666); Instituto de Salud Carlos III—FEDER (CP12/03175 and PI14/01412), (CP09/0063 and PI12/1598); TerCel (RD12/0019/0006); Telemaraton Todos Somos Raros, Todos somos Únicos (IP91-2014)

Resumen

Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies.

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