@misc{10481/105929, year = {2015}, month = {11}, url = {https://hdl.handle.net/10481/105929}, abstract = {Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies.}, organization = {Ministry of Economy and Competitiveness (JCI_2012_12666)}, organization = {Instituto de Salud Carlos III—FEDER (CP12/03175 and PI14/01412), (CP09/0063 and PI12/1598)}, organization = {TerCel (RD12/0019/0006)}, organization = {Telemaraton Todos Somos Raros, Todos somos Únicos (IP91-2014)}, publisher = {Elsevier}, title = {Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells}, doi = {10.1016/j.scr.2015.10.009}, author = {Montes, Rosa and Romero, Tamara and Cabrera, Sonia and Ayllon, Veronica and Lopez-Escamez, Jose Antonio and Ramos-Mejia, Veronica and Real, Pedro Jose}, }