Generation and characterization of the human iPSC line PBMC1-iPS4F1 from adult peripheral blood mononuclear cells Montes, Rosa Romero, Tamara Cabrera, Sonia Ayllon, Veronica Lopez-Escamez, Jose Antonio Ramos Mejía, Verónica Real, Pedro Jose This work was supported by the Postdoctoral Subprogramme Juan de la Cierva founded by the Ministry of Economy and Competitiveness (JCI_2012_12666) to RM; the Instituto de Salud Carlos III—FEDER (CP12/03175 and PI14/01412) to VR-M; (CP09/0063 and PI12/1598) to PJR; TerCel (RD12/0019/0006) and the Telemaraton Todos Somos Raros, Todos somos Únicos (IP91-2014) to PJR. Here we describe the generation and characterization of the human induced pluripotent stem cell (iPSC) line PBMC1-iPS4F1 from peripheral blood mononuclear cells from a healthy female with Spanish background. We used heat sensitive, non-integrative Sendai viruses containing the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc, whose expression was silenced in the established iPSC line. Characterization of the PBMC1-iPS4F1 cell line included analysis of typical pluripotency-associated factors at mRNA and protein level, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation studies. 2025-09-01T10:59:58Z 2025-09-01T10:59:58Z 2015-11 journal article R. Montes et al. / Stem Cell Research 15 (2015) 614–617. https://doi.org/10.1016/j.scr.2015.10.009 https://hdl.handle.net/10481/105929 10.1016/j.scr.2015.10.009 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Elsevier