New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures

Puente Muñoz, Virginia; Paredes Martínez, José Manuel; Resa, Sandra; Vílchez Rienda, José Dámaso; Zitnan, Michal; Miguel Álvarez, Delia; Girón González, María Dolores; Cuerva Carvajal, Juan Manuel; Salto González, Rafael; Crovetto González, Luis

doi:10.1038/s41598-018-38132-y

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Identificadores

URI: https://hdl.handle.net/10481/95783

DOI: 10.1038/s41598-018-38132-y

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Editorial

Springer Nature

Date

2019-02-07

Referencia bibliográfica

Puente-Muñoz, V., Paredes, J.M., Resa, S. et al. New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures. Sci Rep 9, 1659 (2019). https://doi.org/10.1038/s41598-018-38132-y

Sponsorship

Ministerio Español de Ciencia e Innovación (co-financed by FEDER funds) through grants CTQ2014-53598, CTQ2014-55474-C2-2-R; Project FQM2012-790 (Junta de Andalucía, Spain)

Abstract

A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the levels of biothiols associated with ROS generation via single-cell bioimaging.

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