@misc{10481/95783,
year = {2019},
month = {2},
url = {https://hdl.handle.net/10481/95783},
abstract = {A xanthene derivative, Granada Green dinitrobenzene sulfonate (GGDNBS), has been synthesized
to assay cellular oxidative stress based on changes in the concentration of biothiols. The dye is
able to react with biological thiols by a thiolysis reaction that promotes a change in fluorescence
intensity. To demonstrate the usefulness of GGDNBS for in vivo oxidative stress measurements, 661 W
photoreceptor-derived cells were exposed to light to induce ROS generation, and changes in GGDNBS
fluorescence were measured. In these cells, GGDNBS fluorescence was correlated with the biothiol
levels measured by an enzymatic method. Therefore, GGDNBS allows us to monitor changes in the
levels of biothiols associated with ROS generation via single-cell bioimaging.},
organization = {Ministerio Español de Ciencia e Innovación (co-financed by FEDER funds)
through grants CTQ2014-53598, CTQ2014-55474-C2-2-R},
organization = {Project FQM2012-790 (Junta de Andalucía,
Spain)},
publisher = {Springer Nature},
title = {New Thiol-Sensitive Dye Application for Measuring Oxidative Stress in Cell Cultures},
doi = {10.1038/s41598-018-38132-y},
author = {Puente Muñoz, Virginia and Paredes Martínez, José Manuel and Resa, Sandra and Vílchez Rienda, José Dámaso and Zitnan, Michal and Miguel Álvarez, Delia and Girón González, María Dolores and Cuerva Carvajal, Juan Manuel and Salto González, Rafael and Crovetto González, Luis},
}