Microalgae and phytase dietary supplementation improved growth and gut microbiota in juvenile European seabass (Dicentrarchus labrax)
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Peralta-Sánchez, Juan Manuel; Rabelo Ruiz, Miguel; Martín Platero, Antonio Manuel; Vizcaíno, Antonio Jesús; Flores Moreno, Sara; Macías Vidal, Judit; Martos Sitcha, Juan Antonio; Alarcón López, Francisco Javier; Baños, Alberto; Valdivia Martínez, Dolores Eva; Martínez Bueno, ManuelEditorial
BioMed Central
Materia
Arthrospira platensis Dicentrarchus labrax Fish nutrition
Date
2024-09-06Referencia bibliográfica
Peralta Sánchez, J.M. et. al. BMC Genomics 25, 838 (2024). [https://doi.org/10.1186/s12864-024-10760-x]
Sponsorship
Grants PID2021-122287OB-C21 (HYDROALGAE) funded by MCIN/AEI/https://doi.org/10.13039/501100011033 and FEDER, and EQC2019-006380-P to the Service of Experimental Diet; Project entitled “Improvement of the nutritional quality of aquaculture feeds through the incorporation of microalgae hydrolysates enriched in probiotic microorganisms – ALQUABIOTIC” ITC- 20181099, corresponding to the FEDER INNTERCONECTA 2018 call, funded by CDTI (Centre for the Development of Industrial Technology), supported by the Ministry of Science, Innovation and Universities, and co-financed by FEDER funds (European Regional Development Fund) under the 2014–2020 Intelligent Growth Operational Program; European Union’s Horizon 2020 research and innovation program under grant agreement No. 101036768 (NeoGiANT project); Programa Operativo de Empleo Juvenil (Fondo Social Europeo, Junta de Andalucia, Ref 6127)Abstract
Fishmeal and fish oil have been the main sources of protein and fatty acid for aquaculture fish. However, their
increasing price and low sustainability have led the aquafeed industry to seek sustainable alternative feedstuffs
to meet the nutritional requirements of fish and improve their health and performance. Plant proteins have been
successfully used to replace fishery derivatives in aquafeeds, but the presence of anti-nutritional substances is
a potential drawback of this approach. Thus, it has been reported that phytate breakdown can be caused by
feed supplementation with exogenous phytase. The inclusion of microalgae has been proposed to improve gut
functionality in fish fed diets with a high vegetable protein content. The aim of this study was to evaluate the
effect on the growth and gut microbiota of European seabass (Dicentrarchus labrax) juveniles of a diet containing
a blend of microalgae (Arthrospira platensis and Nannochloropsis gaditana) and different concentrations of phytase.
An 83-day feeding trial was conducted, comprising four experimental diets with 2.5% microalgae and 500, 1,000,
2,000, or 10,000 phytase units (FTU)/kg feed and a microalgae- and phytase-free control diet. At the end of the
trial, a significantly increased body weight was observed in fish fed the diet with the highest phytase concentration
(10,000 FTU/kg) versus controls, although the gut bacterial composition did not differ from controls in alpha or beta
diversity with either majority (Weighted UniFrac) or minority bacterial strains (Unweighted UniFrac). In comparison
to the control group, the groups fed diets with 1,000 or 2,000 FTU/kg diets had a lower alpha diversity (Shannon’s
diversity index), while those fed diets with 500 FTU/kg or 1,000 FTU/kg showed distinct clusters in beta diversity
(involving minority ASVs). According to these findings, the diet containing the 2.5% microalgae blend with 10,000
FTU/kg may be useful to increase the aquafeed quality and sustain the growth performance of juvenile European
seabass.