Daidzein and Equol: Ex Vivo and In Silico Approaches Targeting COX-2, iNOS, and the Canonical Inflammasome Signaling Pathway
Metadatos
Mostrar el registro completo del ítemAutor
Márquez-Flores, Yazmín K.; Martínez-Galero, Elizdath; Correa-Basurto, José; Sixto López, Yudibeth; Rodríguez Villegas, Isabel María; Rosillo, María A.; Cárdeno, Ana; Alarcón de la Lastra, CatalinaEditorial
MDPI
Materia
Soy Daidzein Equol
Fecha
2024-05-16Referencia bibliográfica
Márquez-Flores, Y.K.; Martínez-Galero, E.; Correa-Basurto, J.; Sixto-López, Y.; Villegas, I.; Rosillo, M.Á.; Cárdeno, A.; Alarcón-de-la-Lastra, C. Daidzein and Equol: Ex Vivo and In Silico Approaches Targeting COX-2, iNOS, and the Canonical Inflammasome Signaling Pathway. Pharmaceuticals 2024, 17, 647. [https://doi.org/10.3390/ph17050647]
Patrocinador
Spanish Ministerio de Economía y Competitividad, grant number AGL-2017-89342-P; Junta de Andalucía (CTS-259); Instituto Politécnico Nacional, Mexico, BEIFI-SIP-COFAA projects (Márquez-Flores: 20171085, 20181622, 20232041; Correa-Basurto: 20160204); CONACYT (Correa-Basurto: CB- 254600; PDCPN-782)Resumen
Background: The inflammasome is a cytosolic multiprotein complex associated with
multiple autoimmune diseases. Phytochemical compounds in soy (Glycine max) foods, such as
isoflavones, have been reported for their anti-inflammatory properties. Aim: the anti-inflammatory
activity of DZ (daidzein) and EQ (equol) were investigated in an ex vivo model of LPS-stimulated
murine peritoneal macrophages and by molecular docking correlation. Methods: Cells were pretreated
with DZ (25, 50, and 100 μM) or EQ (5, 10, and 25 μM), followed by LPS stimulation. The levels
of PGE2, NO, TNF-α, IL-6, and IL-1β were analyzed by ELISA, whereas the expressions of COX-2,
iNOS, NLRP3, ASC, caspase 1, and IL-18 were measured by Western blotting. Also, the potential
for transcriptional modulation by targeting NF-κB, COX-2, iNOS, NLRP3, ASC, and caspase 1 was
investigated by molecular docking. Results: The anti-inflammatory responses observed may be due
to the modulation of NF-κB due to the binding of DZ or EQ, which is translated into decreased TNF-α,
COX-2, iNOS, NLRP3, and ASC levels. Conclusion: This study establishes that DZ and EQ inhibit
LPS-induced inflammatory responses in peritoneal murine macrophages via down-regulation of NO
and PGE2 generation, as well as the inhibition of the canonical inflammasome pathway, regulating
NLRP3, and consequently decreasing IL-1β and IL-18 activation.