@misc{10481/93416,
year = {2024},
month = {5},
url = {https://hdl.handle.net/10481/93416},
abstract = {Background: The inflammasome is a cytosolic multiprotein complex associated with
multiple autoimmune diseases. Phytochemical compounds in soy (Glycine max) foods, such as
isoflavones, have been reported for their anti-inflammatory properties. Aim: the anti-inflammatory
activity of DZ (daidzein) and EQ (equol) were investigated in an ex vivo model of LPS-stimulated
murine peritoneal macrophages and by molecular docking correlation. Methods: Cells were pretreated
with DZ (25, 50, and 100 μM) or EQ (5, 10, and 25 μM), followed by LPS stimulation. The levels
of PGE2, NO, TNF-α, IL-6, and IL-1β were analyzed by ELISA, whereas the expressions of COX-2,
iNOS, NLRP3, ASC, caspase 1, and IL-18 were measured by Western blotting. Also, the potential
for transcriptional modulation by targeting NF-κB, COX-2, iNOS, NLRP3, ASC, and caspase 1 was
investigated by molecular docking. Results: The anti-inflammatory responses observed may be due
to the modulation of NF-κB due to the binding of DZ or EQ, which is translated into decreased TNF-α,
COX-2, iNOS, NLRP3, and ASC levels. Conclusion: This study establishes that DZ and EQ inhibit
LPS-induced inflammatory responses in peritoneal murine macrophages via down-regulation of NO
and PGE2 generation, as well as the inhibition of the canonical inflammasome pathway, regulating
NLRP3, and consequently decreasing IL-1β and IL-18 activation.},
organization = {Spanish Ministerio de Economía
y Competitividad, grant number AGL-2017-89342-P},
organization = {Junta de Andalucía (CTS-259)},
organization = {Instituto Politécnico Nacional, Mexico, BEIFI-SIP-COFAA projects (Márquez-Flores:
20171085, 20181622, 20232041},
organization = {Correa-Basurto: 20160204)},
organization = {CONACYT (Correa-Basurto: CB-
254600; PDCPN-782)},
publisher = {MDPI},
keywords = {Soy},
keywords = {Daidzein},
keywords = {Equol},
title = {Daidzein and Equol: Ex Vivo and In Silico Approaches Targeting COX-2, iNOS, and the Canonical Inflammasome Signaling Pathway},
doi = {10.3390/ph17050647},
author = {Márquez-Flores, Yazmín K. and Martínez-Galero, Elizdath and Correa-Basurto, José and Sixto López, Yudibeth and Rodríguez Villegas, Isabel María and Rosillo, María A. and Cárdeno, Ana and Alarcón de la Lastra, Catalina},
}