A panel of phenotypically and genotypically diverse bioluminescent:fluorescent Trypanosoma cruzi strains as a resource for Chagas disease research
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Olmo Arévalo, Francisco; Jayawardhana, Shiromani; A. Khan, Archie; C. Langston, Henry; Fortes Francisco, Amanda; L. Atherton, Richard; I. Ward, Alex; C. Taylor, Martin; M. Kelly, John; D. Lewis, MichaelEditorial
PLOS
Fecha
2024-05-31Referencia bibliográfica
Olmo, F. et. al. PLoS Negl Trop Dis 18(5): e0012106. [https://doi.org/10.1371/journal.pntd.0012106]
Patrocinador
UK Medical Research Council (MRC) grants MR/T015969/1 to J.M.K. and MR/R021430/1 to M.D.L. AIW was in receipt of an MRC LID (DTP) Studentship (MR/ N013638/1); Department for International Development (DFID), UK; Federal Ministry of Education and Research (BMBF) through KfW, Germany; Me´decins sans Frontières (MSF) InternationalResumen
Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that displays considerable
genetic diversity. Infections result in a range of pathological outcomes, and different
strains can exhibit a wide spectrum of anti-parasitic drug tolerance. The genetic determinants
of infectivity, virulence and therapeutic susceptibility remain largely unknown. As
experimental tools to address these issues, we have generated a panel of bioluminescent:
fluorescent parasite strains that cover the diversity of the T. cruzi species. These reporters
allow spatio-temporal infection dynamics in murine models to be monitored in a non-invasive
manner by in vivo imaging, provide a capability to detect rare infection foci at single-cell resolution,
and represent a valuable resource for investigating virulence and host:parasite interactions
at a mechanistic level. Importantly, these parasite reporter strains can also
contribute to the Chagas disease drug screening cascade by ensuring that candidate compounds
have pan-species in vivo activity prior to being advanced into clinical testing. The
parasite strains described in this paper are available on request.