@misc{10481/93349,
year = {2024},
month = {5},
url = {https://hdl.handle.net/10481/93349},
abstract = {Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that displays considerable
genetic diversity. Infections result in a range of pathological outcomes, and different
strains can exhibit a wide spectrum of anti-parasitic drug tolerance. The genetic determinants
of infectivity, virulence and therapeutic susceptibility remain largely unknown. As
experimental tools to address these issues, we have generated a panel of bioluminescent:
fluorescent parasite strains that cover the diversity of the T. cruzi species. These reporters
allow spatio-temporal infection dynamics in murine models to be monitored in a non-invasive
manner by in vivo imaging, provide a capability to detect rare infection foci at single-cell resolution,
and represent a valuable resource for investigating virulence and host:parasite interactions
at a mechanistic level. Importantly, these parasite reporter strains can also
contribute to the Chagas disease drug screening cascade by ensuring that candidate compounds
have pan-species in vivo activity prior to being advanced into clinical testing. The
parasite strains described in this paper are available on request.},
organization = {UK Medical
Research Council (MRC) grants MR/T015969/1 to
J.M.K. and MR/R021430/1 to M.D.L. AIW was in
receipt of an MRC LID (DTP) Studentship (MR/
N013638/1)},
organization = {Department for
International Development (DFID), UK},
organization = {Federal
Ministry of Education and Research (BMBF)
through KfW, Germany},
organization = {Me´decins sans
Frontières (MSF) International},
publisher = {PLOS},
title = {A panel of phenotypically and genotypically diverse bioluminescent:fluorescent Trypanosoma cruzi strains as a resource for Chagas disease research},
doi = {10.1371/journal.pntd.0012106},
author = {Olmo Arévalo, Francisco and Jayawardhana, Shiromani and A. Khan, Archie and C. Langston, Henry and Fortes Francisco, Amanda and L. Atherton, Richard and I. Ward, Alex and C. Taylor, Martin and M. Kelly, John and D. Lewis, Michael},
}