Identification of volatile organic compounds in extremophilic bacteria and their effective use in biocontrol of postharvest fungal phytopathogens
Metadatos
Afficher la notice complèteAuteur
Toral Navarro, Laura; Rodríguez González, Miguel Ángel; Martínez-Checa Barrero, Fernando José; Montaño, Alfredo; Cortés-Delgado, Amparo; Smolinska, Agnieszka; Llamas Company, Inmaculada; Sampedro Quesada, María InmaculadaEditorial
Frontiers Media
Materia
Volatile compounds Antifungal activity Biocontrol Fungal phytopathogens Postharvest diseases
Date
2021-11-12Referencia bibliográfica
Toral L, Rodríguez M, Martínez-Checa F, Montaño A, Cortés-Delgado A, Smolinska A, Llamas I and Sampedro I (2021) Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens. Front. Microbiol. 12:773092. doi: 10.3389/fmicb.2021.773092
Patrocinador
Spanish Ministry of the Economy and Competitiveness (PID2019-106704RB-100/ AEI/10.13039/501100011033); European Project for Industrial Doctorates ‘H2020’ (UGR-Ref. 4726); Junta de Andalucía B-AGR-222- UGR20; ERDF A way of making EuropeRésumé
Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for
20–25% of production losses. Volatile organic compounds (VOCs) have been gaining
importance in the food industry as a safe and ecofriendly alternative to pesticides for
combating these phytopathogenic fungi. In this study, we analysed the ability of some
VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas,
Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis
cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and
Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/
MS and 87 volatile compounds were identified, in particular acetoin, acetic acid,
2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth
inhibition assays and in vivo experiments using cherry fruits showed that the best producers
of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis
Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa,
which highlights the potential of these strains to control postharvest diseases. Transmission
electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal
activity which led to an intense degeneration of cellular components of mycelium and
cell death.