Promastigote EPS secretion and haptomonad biofilm formation as evolutionary adaptations of trypanosomatid parasites for colonizing honeybee hosts
Metadatos
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Carreira de Paula, Jessica; García Olmedo, Pedro; Gómez Moracho, Tamara; Buendía Abad, María; Higes, Mariano; Martín Hernández, Raquel; Osuna Carrillo De Albornoz, Antonio; Pablos Torró, Luis Miguel deEditorial
Springer Nature
Fecha
2024-03-21Referencia bibliográfica
Carreira de Paula, J., García Olmedo, P., Gómez-Moracho, T. et al. Promastigote EPS secretion and haptomonad biofilm formation as evolutionary adaptations of trypanosomatid parasites for colonizing honeybee hosts. npj Biofilms Microbiomes 10, 27 (2024). https://doi.org/10.1038/s41522-024-00492-x
Patrocinador
Spanish Programme for Knowledge Generation and Scientific and Technological Strengthening of the R+D+I System, grant PID2021-126938OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF/EU"; Grant PGC2018-098929-A-I00 funded by MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe"; Maria Zambrano postdoctoral fellowship program of the Spanish Ministry of Science, Innovation and Universities (RD289/2021); Doctoral Program in Fundamental and Systems Biology (University of Granada, UGR)Resumen
Bees are major pollinators involved in the maintenance of all terrestrial ecosystems. Biotic and abiotic
factors placing these insects at risk is a research priority for ecological and agricultural sustainability.
Parasites are one of the key players of this global decline and the study of their mechanisms of action is
essential to control honeybee colony losses. Trypanosomatid parasites and particularly the Lotmaria
passim are widely spread in honeybees, however their lifestyle is poorly understood. In this work, we
show how these parasites are able to differentiate into a new parasitic lifestyle: the trypanosomatid
biofilms. Using different microscopic techniques, we demonstrated that the secretion of Extracellular
Polymeric Substances by free-swimming unicellular promastigote forms is a prerequisite for the
generation and adherence of multicellular biofilms to solid surfaces in vitro and in vivo. Moreover,
compared to human-infective trypanosomatid parasites our study shows how trypanosomatid
parasites of honeybees increases their resistance and thus resilience to drastic changes in
environmental conditions such as ultralow temperatures and hypoosmotic shock, which would
explain their success thriving within or outside their hosts. These results set up the basis for the
understanding of the success of this group of parasites in nature and to unveil the impact of such
pathogens in honeybees, a keystones species in most terrestrial ecosystems.