@misc{10481/92043,
year = {2024},
month = {3},
url = {https://hdl.handle.net/10481/92043},
abstract = {Bees are major pollinators involved in the maintenance of all terrestrial ecosystems. Biotic and abiotic
factors placing these insects at risk is a research priority for ecological and agricultural sustainability.
Parasites are one of the key players of this global decline and the study of their mechanisms of action is
essential to control honeybee colony losses. Trypanosomatid parasites and particularly the Lotmaria
passim are widely spread in honeybees, however their lifestyle is poorly understood. In this work, we
show how these parasites are able to differentiate into a new parasitic lifestyle: the trypanosomatid
biofilms. Using different microscopic techniques, we demonstrated that the secretion of Extracellular
Polymeric Substances by free-swimming unicellular promastigote forms is a prerequisite for the
generation and adherence of multicellular biofilms to solid surfaces in vitro and in vivo. Moreover,
compared to human-infective trypanosomatid parasites our study shows how trypanosomatid
parasites of honeybees increases their resistance and thus resilience to drastic changes in
environmental conditions such as ultralow temperatures and hypoosmotic shock, which would
explain their success thriving within or outside their hosts. These results set up the basis for the
understanding of the success of this group of parasites in nature and to unveil the impact of such
pathogens in honeybees, a keystones species in most terrestrial ecosystems.},
organization = {Spanish Programme for Knowledge Generation and
Scientific and Technological Strengthening of the R+D+I System, grant
PID2021-126938OB-I00 funded by MCIN/AEI/10.13039/501100011033
and by "ERDF/EU"},
organization = {Grant PGC2018-098929-A-I00 funded by
MCIN/AEI/10.13039/501100011033 and by "ERDF A way of making Europe"},
organization = {Maria Zambrano postdoctoral fellowship
program of the Spanish Ministry of Science, Innovation and Universities
(RD289/2021)},
organization = {Doctoral Program in Fundamental and Systems Biology
(University of Granada, UGR)},
publisher = {Springer Nature},
title = {Promastigote EPS secretion and haptomonad biofilm formation as evolutionary adaptations of trypanosomatid parasites for colonizing honeybee hosts},
doi = {10.1038/s41522-024-00492-x},
author = {Carreira de Paula, Jessica and García Olmedo, Pedro and Gómez Moracho, Tamara and Buendía Abad, María and Higes, Mariano and Martín Hernández, Raquel and Osuna Carrillo De Albornoz, Antonio and Pablos Torró, Luis Miguel de},
}