Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study
Metadatos
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Rasheed Al-Bakri, Sarmad Muayad; Magán Fernández, Antonio; Galindo Moreno, Pablo Antonio; O'Valle Ravassa, Francisco Javier; Martín Morales, Natividad; Padial Molina, Miguel; Mesa Aguado, Francisco LuisEditorial
Wiley
Materia
Immunohistochemistry Microscopy Neutrophils
Fecha
2024-03-18Referencia bibliográfica
Al-Bakri SMR, Magan-Fernandez A, Galindo-Moreno P, et al. Detection and comparison of neutrophil extracellular traps in tissue samples of periimplantitis, periodontitis, and healthy patients: A pilot study. Clin Implant Dent Relat Res. 2024;1‐11. doi:10.1111/cid.13325
Patrocinador
Junta de Andalucía, Spain. Research Groups #CTS 583, #CTS-1028, and #CTS-138; Universidad de Granada / CBUA (Funding for open access charge)Resumen
Objective: The aim of this study was to detect and compare the tissular expression
of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of
patients with peri-implantitis, periodontitis, and controls.
Materials and Methods: An observational study was performed on patients with
peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical
examinations were performed on each participant. Tissue samples were collected
during tooth/implant extraction for clinical reasons. Electron microscopy analysis,
Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence
(citrullinated H3 and myeloperoxidase) techniques were performed to detect NETrelated
structures and the degree of connective tissue destruction, between the
study groups.
Results: Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis,
and 10 controls, with a total of 51 implants, 26 periodontal teeth, and
10 control teeth. Neutrophil release of nuclear content was observed in transmission
electron microscopy. Immunohistochemical analysis showed a greater CD15 expression
in both peri-implantitis and periodontitis compared to controls (p < 0.001), and
peri-implantitis presented lower levels of connective tissue and collagen compared to
both periodontitis (p = 0.044; p < 0.001) and controls (p < 0.001). Immunofluorescence
showed greater citH3 expression in peri-implantitis than the one found in both
periodontitis (p = 0.003) and controls (p = 0.048). Conclusions: A greater presence and involvement of neutrophils, as well as a greater
connective tissue destruction were observed in cases of peri-implantitis. A higher
expression of NET-related markers was found in mucosal samples of peri-implantitis
compared to periodontitis and controls.