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Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study
dc.contributor.author | Rasheed Al-Bakri, Sarmad Muayad | |
dc.contributor.author | Magán Fernández, Antonio | |
dc.contributor.author | Galindo Moreno, Pablo Antonio | |
dc.contributor.author | O'Valle Ravassa, Francisco Javier | |
dc.contributor.author | Martín Morales, Natividad | |
dc.contributor.author | Padial Molina, Miguel | |
dc.contributor.author | Mesa Aguado, Francisco Luis | |
dc.date.accessioned | 2024-05-14T08:03:35Z | |
dc.date.available | 2024-05-14T08:03:35Z | |
dc.date.issued | 2024-03-18 | |
dc.identifier.citation | Al-Bakri SMR, Magan-Fernandez A, Galindo-Moreno P, et al. Detection and comparison of neutrophil extracellular traps in tissue samples of periimplantitis, periodontitis, and healthy patients: A pilot study. Clin Implant Dent Relat Res. 2024;1‐11. doi:10.1111/cid.13325 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/91739 | |
dc.description.abstract | Objective: The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri-implant and periodontal samples of patients with peri-implantitis, periodontitis, and controls. Materials and Methods: An observational study was performed on patients with peri-implantitis, periodontitis, and controls. Peri-implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro-Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NETrelated structures and the degree of connective tissue destruction, between the study groups. Results: Sixty-four patients were included in the study: 28 peri-implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri-implantitis and periodontitis compared to controls (p < 0.001), and peri-implantitis presented lower levels of connective tissue and collagen compared to both periodontitis (p = 0.044; p < 0.001) and controls (p < 0.001). Immunofluorescence showed greater citH3 expression in peri-implantitis than the one found in both periodontitis (p = 0.003) and controls (p = 0.048). Conclusions: A greater presence and involvement of neutrophils, as well as a greater connective tissue destruction were observed in cases of peri-implantitis. A higher expression of NET-related markers was found in mucosal samples of peri-implantitis compared to periodontitis and controls. | es_ES |
dc.description.sponsorship | Junta de Andalucía, Spain. Research Groups #CTS 583, #CTS-1028, and #CTS-138 | es_ES |
dc.description.sponsorship | Universidad de Granada / CBUA (Funding for open access charge) | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Immunohistochemistry | es_ES |
dc.subject | Microscopy | es_ES |
dc.subject | Neutrophils | es_ES |
dc.title | Detection and comparison of neutrophil extracellular traps in tissue samples of peri-implantitis, periodontitis, and healthy patients: A pilot study | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1111/cid.13325 | |
dc.type.hasVersion | VoR | es_ES |