Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6
Metadatos
Mostrar el registro completo del ítemAutor
Fernández, F.; Gutiérrez Fernández, José; Sorlozano Puerto, Antonio; Romero, J.M.; Soto, M.J.; Ruiz-Cabello, FranciscoEditorial
Elsevier
Materia
Human herpes virus 6 (HHV-6) PCR Real-time PCR
Fecha
2006Referencia bibliográfica
Fernández F, Gutiérrez J, Sorlózano A, Romero JM, Soto MJ, Ruiz-Cabello F. Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6. Microbiol Res. 2006;161(2):158-63. https://doi.org/10.1016/j.micres.2005.07.007
Resumen
A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.