@misc{10481/90009,
year = {2006},
url = {https://hdl.handle.net/10481/90009},
abstract = {A comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.},
publisher = {Elsevier},
keywords = {Human herpes virus 6 (HHV-6)},
keywords = {PCR},
keywords = {Real-time PCR},
title = {Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6},
doi = {10.1016/j.micres.2005.07.007},
author = {Fernández, F. and Gutiérrez Fernández, José and Sorlozano Puerto, Antonio and Romero, J.M. and Soto, M.J. and Ruiz-Cabello, Francisco},
}