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Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding
dc.contributor.author | Delgado-Gonzalez, Antonio | |
dc.contributor.author | Laz Ruiz, José Antonio | |
dc.contributor.author | Cano Cortes, María Victoria | |
dc.contributor.author | Díaz Mochón, Juan José | |
dc.contributor.author | Sánchez Martín, Rosario María | |
dc.date.accessioned | 2024-02-07T11:41:47Z | |
dc.date.available | 2024-02-07T11:41:47Z | |
dc.date.issued | 2022-07-22 | |
dc.identifier.citation | Delgado Gonzalez, A; Laz Ruiz, JA; Cano Cortés, MV; Huang Y.W.; Gonzalez VD; Díaz Mochon JJ; Fantl WJ*; Sanchez Martin, RM*, Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding, Anal. Chem. 2022, 94, 30, 10626–10635 https://doi.org/10.1021/acs.analchem.2c00795 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/88581 | |
dc.description.abstract | Cell barcoding and pooling are essential to reduce technical variability in multiplex technologies. While fluorescent barcoding is common in flow cytometry, current mass-tag barcoding methods are unsuitable for live cells. This study presents fluorescent palladium-based nanotrackers for live cell barcoding, compatible with flow and mass cytometry, enabling long-term drug studies with consistent labeling. | es_ES |
dc.description.sponsorship | This research was supported by funding from the Spanish Ministry of Economy and Competitiveness (BIO2016-80519, PID2019.110987RB.I00); the Health Institute Carlos III (DTS18/00121); the Andalusian Regional Government-FEDER (PT18-TP-4160, B-FQM-475-UGR18, A-FQM-760-UGR20); the Department of Defense (W81XWH-12-1-0591); NCI (1R01CA234553, R21CA231280); the 2019 Cancer Innovation Award, supported by the Stanford Cancer Institute, an NCI-designated Comprehensive Cancer Center; BRCA Foundation and the V Foundation for Cancer Research; a gift from the Gray Foundation; the Department of Urology at Stanford University; NHLBI (P01HL10879709); NIAID (U19AI057229); and a PICI Bedside to Bench grant. R.M.S.-M., J.J.D.-M., M.V.C.-C., and J.A.L.-R. are members of the network NANOCARE (RED2018-102469-T) funded by the Spanish State Research Agency. W.J.F. thanks the BRCA Foundation and the V Foundation for Cancer Research, a gift from the Gray Foundation and the Department of Urology at Stanford University. A.D.-G. thanks the Spanish Ministry of Education for PhD funding (scholarship FPU14/02181), the University of Granada for postdoctoral research contract, and the Fundación Alfonso Martín Escudero for his postdoctoral fellowship. J.A.L.-R. thanks the Fundación Benéfica Anticancer San Francisco Javier y Santa Candida for PhD funding. M.V.C.-C. thanks the Andalusian Regional Government for her postdoctoral fellowship (POSTDOC_21_00118). The authors thank the technical support from the Cytometry unit of GENYO. The graphical abstract and Figures 5a and 6a have been created with BioRender.com. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | ACS Publications | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | doi.org/10.1021/acs.analchem.2c00795 | |
dc.type.hasVersion | VoR | es_ES |