Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding

Identificadores
URI: https://hdl.handle.net/10481/88581
Exportar
RISMendeleyBibtex
Estadísticas
Ver Estadísticas de uso
Metadatos
Mostrar el registro completo del ítem
Autor
Delgado-Gonzalez, Antonio; Laz Ruiz, José Antonio; Cano Cortes, María Victoria; Díaz Mochón, Juan José; Sánchez Martín, Rosario María
Editorial
ACS Publications
Fecha
2022-07-22
Referencia bibliográfica
Delgado Gonzalez, A; Laz Ruiz, JA; Cano Cortés, MV; Huang Y.W.; Gonzalez VD; Díaz Mochon JJ; Fantl WJ*; Sanchez Martin, RM*, Hybrid Fluorescent Mass-Tag Nanotrackers as Universal Reagents for Long-Term Live-Cell Barcoding, Anal. Chem. 2022, 94, 30, 10626–10635 https://doi.org/10.1021/acs.analchem.2c00795
Patrocinador
This research was supported by funding from the Spanish Ministry of Economy and Competitiveness (BIO2016-80519, PID2019.110987RB.I00); the Health Institute Carlos III (DTS18/00121); the Andalusian Regional Government-FEDER (PT18-TP-4160, B-FQM-475-UGR18, A-FQM-760-UGR20); the Department of Defense (W81XWH-12-1-0591); NCI (1R01CA234553, R21CA231280); the 2019 Cancer Innovation Award, supported by the Stanford Cancer Institute, an NCI-designated Comprehensive Cancer Center; BRCA Foundation and the V Foundation for Cancer Research; a gift from the Gray Foundation; the Department of Urology at Stanford University; NHLBI (P01HL10879709); NIAID (U19AI057229); and a PICI Bedside to Bench grant. R.M.S.-M., J.J.D.-M., M.V.C.-C., and J.A.L.-R. are members of the network NANOCARE (RED2018-102469-T) funded by the Spanish State Research Agency. W.J.F. thanks the BRCA Foundation and the V Foundation for Cancer Research, a gift from the Gray Foundation and the Department of Urology at Stanford University. A.D.-G. thanks the Spanish Ministry of Education for PhD funding (scholarship FPU14/02181), the University of Granada for postdoctoral research contract, and the Fundación Alfonso Martín Escudero for his postdoctoral fellowship. J.A.L.-R. thanks the Fundación Benéfica Anticancer San Francisco Javier y Santa Candida for PhD funding. M.V.C.-C. thanks the Andalusian Regional Government for her postdoctoral fellowship (POSTDOC_21_00118). The authors thank the technical support from the Cytometry unit of GENYO. The graphical abstract and Figures 5a and 6a have been created with BioRender.com.
Resumen
Cell barcoding and pooling are essential to reduce technical variability in multiplex technologies. While fluorescent barcoding is common in flow cytometry, current mass-tag barcoding methods are unsuitable for live cells. This study presents fluorescent palladium-based nanotrackers for live cell barcoding, compatible with flow and mass cytometry, enabling long-term drug studies with consistent labeling.
Colecciones
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional