Direct detection of alpha satellite -	DNA with single-base resolution by using abasic Peptide Nucleic Acids and Fluorescent in situ Hybridization

Sánchez Martín, Rosario María

doi:https://doi.org/10.1016/j.bios.2022.114770

Articulo Principal (1.140Mb)

Identificadores

URI: https://hdl.handle.net/10481/88539

DOI: https://doi.org/10.1016/j.bios.2022.114770

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Editorial

Elsevier

Fecha

2023-01-01

Referencia bibliográfica

Robles-Remacho A, Luque-Gonzalez MA, López-Delgado FJ, Guardia-Monteagudo JJ, Fara MA, Pernagallo S, Sanchez-Martin RM, Diaz-Mochon JJ. Direct detection of alpha satellite DNA with single-base resolution by using abasic Peptide Nucleic Acids and Fluorescent in situ Hybridization. Biosens Bioelectron. 2023 Jan 1;219:114770. doi: 10.1016/j.bios.2022.114770. Epub 2022 Oct 7. PMID: 36270082.

Patrocinador

This research was supported by the Spanish Ministry of Economy and Competitiveness (grant number BIO2016-80519-R, PID2019.110987RB.I00); the Andalusian Regional Government-FEDER (PT18-TP-4160, B-FQM-475-UGR18, A-FQM-760-UGR20); the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie actions (MSCA-RISE-101007934). The authors are members of the NANOCARE network (RED2018-102469-T) funded by the State Investigation Agency. ARR thanks the Spanish Ministry of Education for PhD funding (scholarship FPU15/06418) and the University of Granada for postdoctoral research. FJ López-Delgado thanks the Spanish Ministry of Economy and Competitiveness for the Torres Quevedo fellowship (PTQ-16-08597). These studies were approved and supported by DESTINA Genomics Ltd. Schemes in Graphical abstract and Fig. 1 have been partially created using BioRender.com. We thank Raquel Marrero-Díaz for her valuable support in the microscopy analyses.

Resumen

As the importance of understanding genomic variations in repetitive sequences grows, the lack of validation techniques for Next-Generation Sequencing data remains a challenge, particularly for Single-Nucleotide Variations (SNVs). We introduce chemFISH, a dynamic chemistry-based method utilizing abasic Peptide Nucleic Acid (PNA) probes to detect α-satellite DNA with single-base resolution. This innovative approach offers rapid validation of SNVs and potential for novel nucleic acid detection assays.

Colecciones

CTS987 - Artículos

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