Direct detection of alpha satellite - DNA with single-base resolution by using abasic Peptide Nucleic Acids and Fluorescent in situ Hybridization Sánchez Martín, Rosario María As the importance of understanding genomic variations in repetitive sequences grows, the lack of validation techniques for Next-Generation Sequencing data remains a challenge, particularly for Single-Nucleotide Variations (SNVs). We introduce chemFISH, a dynamic chemistry-based method utilizing abasic Peptide Nucleic Acid (PNA) probes to detect α-satellite DNA with single-base resolution. This innovative approach offers rapid validation of SNVs and potential for novel nucleic acid detection assays. 2024-02-07T10:19:47Z 2024-02-07T10:19:47Z 2023-01-01 journal article Robles-Remacho A, Luque-Gonzalez MA, López-Delgado FJ, Guardia-Monteagudo JJ, Fara MA, Pernagallo S, Sanchez-Martin RM, Diaz-Mochon JJ. Direct detection of alpha satellite DNA with single-base resolution by using abasic Peptide Nucleic Acids and Fluorescent in situ Hybridization. Biosens Bioelectron. 2023 Jan 1;219:114770. doi: 10.1016/j.bios.2022.114770. Epub 2022 Oct 7. PMID: 36270082. https://hdl.handle.net/10481/88539 https://doi.org/10.1016/j.bios.2022.114770 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier