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L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode
dc.contributor.author | Rodríguez Heras, Sara | |
dc.contributor.author | Thomas, M.C. | |
dc.contributor.author | García Cañadas, Marta | |
dc.contributor.author | García Pérez, José Luis | |
dc.contributor.author | López López, Manuel Carlos | |
dc.date.accessioned | 2024-02-06T12:45:29Z | |
dc.date.available | 2024-02-06T12:45:29Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Heras SR, Thomas MC, García-Canadas M, de Felipe P, García-Pérez JL, Ryan MD, López MC. L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode. Cell Mol Life Sci. 2006 Jun;63(12):1449-60. doi: 10.1007/s00018-006-6038-2. | es_ES |
dc.identifier.uri | https://hdl.handle.net/10481/88462 | |
dc.description.abstract | A comparative analysis of 40 Trypanosoma cruzi L1Tc elements showed that the 2A self-cleaving sequence described in viruses is present in them. Of these elements, 72% maintain the canonical 2A motif (DxExNPGP). A high percentage has a conserved point mutation within the motif that has not been previously described. In vitro and in vivo expression of reporter polyproteins showed that the L1Tc2A sequence is functional. Mutations within certain L1Tc2A sequences affect the efficiency of the cleavage. The data indicate that the L1Tc2A sequence may be influencing the L1Tc enzymatic machinery determining the composition and level of the translated products. The residues located immediately upstream of the 2A consensus sequence increase the cleaving efficiency and appear to stabilize the relative amount of translated products. | es_ES |
dc.description.sponsorship | This work was supported by BMC2003-00834 from Plan Nacional I+D+I (MEC); PAI ref CVI 1227 (Junta de Andalucia) and RICET C03-04 from FIS (MSC) Spain. S. R. Heras and M. García were supported by a MEC Predoctoral Fellowship (FPU and FPI, respectively), Fundación Ramón Areces Predoctoral Fellowship supported to J.L. García-Pérez. The support of the Wellcome Trust and BBSRC is also gratefully acknowledged (P. de Felipe and M. Ryan). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.identifier.doi | 10.1007/s00018-006-6038-2 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |