L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode
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Rodríguez Heras, Sara; Thomas, M.C.; García Cañadas, Marta; García Pérez, José Luis; López López, Manuel CarlosEditorial
Springer Nature
Fecha
2006Referencia bibliográfica
Heras SR, Thomas MC, García-Canadas M, de Felipe P, García-Pérez JL, Ryan MD, López MC. L1Tc non-LTR retrotransposons from Trypanosoma cruzi contain a functional viral-like self-cleaving 2A sequence in frame with the active proteins they encode. Cell Mol Life Sci. 2006 Jun;63(12):1449-60. doi: 10.1007/s00018-006-6038-2.
Patrocinador
This work was supported by BMC2003-00834 from Plan Nacional I+D+I (MEC); PAI ref CVI 1227 (Junta de Andalucia) and RICET C03-04 from FIS (MSC) Spain. S. R. Heras and M. García were supported by a MEC Predoctoral Fellowship (FPU and FPI, respectively), Fundación Ramón Areces Predoctoral Fellowship supported to J.L. García-Pérez. The support of the Wellcome Trust and BBSRC is also gratefully acknowledged (P. de Felipe and M. Ryan).Resumen
A comparative analysis of 40 Trypanosoma
cruzi L1Tc elements showed that the 2A self-cleaving sequence
described in viruses is present in them. Of these
elements, 72% maintain the canonical 2A motif (DxExNPGP).
A high percentage has a conserved point mutation
within the motif that has not been previously described.
In vitro and in vivo expression of reporter polyproteins
showed that the L1Tc2A sequence is functional. Mutations
within certain L1Tc2A sequences affect the efficiency
of the cleavage. The data indicate that the L1Tc2A
sequence may be influencing the L1Tc enzymatic machinery
determining the composition and level of the
translated products. The residues located immediately
upstream of the 2A consensus sequence increase the
cleaving efficiency and appear to stabilize the relative
amount of translated products.