Establishment of a Sonotrode Ultrasound-Assisted Extraction of Phenolic Compounds from Apple Pomace
Metadatos
Mostrar el registro completo del ítemAutor
Razola Díaz, María del Carmen; Aznar Ramos, María José; Guerra Hernández, Eduardo Jesús; García Villanova Ruiz, Belén; Gómez Caravaca, Ana María; Verardo, VitoEditorial
MDPI
Materia
HPLC-MS Malus Antioxidant activity Amygdalin By-product Waste revalorization
Fecha
2022-11-25Referencia bibliográfica
Razola-Díaz, M.d.C... [et al.]. Establishment of a Sonotrode Ultrasound-Assisted Extraction of Phenolic Compounds from Apple Pomace. Foods 2022, 11, 3809. [https://doi.org/10.3390/foods11233809]
Patrocinador
SHEALTHY project - European Union's Horizon 2020 research and innovation program 817936Resumen
Apple pomace is the main by-product from apple processing in the juice industry and is
considered a source of polyphenols with several health bioactivities. Thus, this research focuses on
the establishment of the ultrasound-assisted extraction of total phenolic compounds, focusing on
phloretin and phloridzin, with high antioxidant activity from apple pomace, using a sonotrode. We
used a Box–Behnken design of 15 experiments with 3 independent factors (ethanol (%), time (min)
and amplitude (%)). The responses evaluated were the sum of phenolic compounds, phloretin and
phloridzin measured by HPLC–MS-ESI-TOF, and antioxidant activity measured by DPPH, ABTS
and FRAP. The validity of the model was confirmed by ANOVA. Further, it was carried out using
a comparison between different apple pomaces with or without seeds extracted by the optimal
conditions. Phloretin and phloridzin accounted for 7 to 32% of the total phenolic compounds in
the apple pomaces. Among all the apple pomace analyzed, that of the variety Gala had the highest
phenolic content and antioxidant activity. The presence of the cyanogenic compound amygdalin was
detected in apple pomaces that contained seeds accompanied with a higher content of phloretin and
phloridzin but a lower content of flavan-3-ols.