Pitx2 Differentially Regulates the Distinct Phases of Myogenic Program and Delineates Satellite Cell Lineages During Muscle Development
Metadatos
Mostrar el registro completo del ítemAutor
Ramírez de Acuña, Felicitas; Hernández Torres, Francisco; Rodríguez Outeiriño, Lara; Domínguez, Jorge N.; Matías Valiente, Lidia; Sánchez Fernández, Cristina; Franco, Diego; Aranega, Amelia E.Editorial
Frontiers
Materia
Pitx2 Myogenic precursors Satellite cells Myogenesis Somites
Fecha
2022-07-06Referencia bibliográfica
Ramírez de Acuña F... [et al.] (2022) Pitx2 Differentially Regulates the Distinct Phases of Myogenic Program and Delineates Satellite Cell Lineages During Muscle Development. Front. Cell Dev. Biol. 10:940622. doi: [10.3389/fcell.2022.940622]
Patrocinador
BFU2015-67131 (Spanish Ministery of Economy and Competitiveness); PID2019- 107492GB-100 (Spanish Ministry of Science and Innovation)Resumen
The knowledge of the molecular mechanisms that regulate embryonic myogenesis from
early myogenic progenitors to myoblasts, as well as the emergence of adult satellite stem
cells (SCs) during development, are key concepts to understanding the genesis and
regenerative abilities of the skeletal muscle. Several previous pieces of evidence have
revealed that the transcription factor Pitx2 might be a player within the molecular pathways
controlling somite-derived muscle progenitors’ fate and SC behavior. However, the role
exerted by Pitx2 in the progression from myogenic progenitors to myoblasts including SC
precursors remains unsolved. Here, we show that Pitx2 inactivation in uncommitted early
myogenic precursors diminished cell proliferation and migration leading to muscle
hypotrophy and a low number of SCs with decreased myogenic differentiation
potential. However, the loss of Pitx2 in committed myogenic precursors gave rise to
normal muscles with standard amounts of SCs exhibiting high levels of Pax7 expression.
This SC population includes few MYF5+ SC-primed but increased amount of less
proliferative miR-106b+cells, and display myogenic differentiation defects failing to
undergo proper muscle regeneration. Overall our results demonstrate that Pitx2 is
required in uncommitted myogenic progenitors but it is dispensable in committed
precursors for proper myogenesis and reveal a role for this transcription factor in the
generation of diverse SC subpopulations.