Mostrar el registro sencillo del ítem
Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p
dc.contributor.author | Rocha Bartolomeu, Ariane | |
dc.contributor.author | Griñán Lisón, Carmen | |
dc.contributor.author | Marchal Corrales, Juan Antonio | |
dc.contributor.author | García Chaves, María Ángel | |
dc.date.accessioned | 2022-06-30T11:45:49Z | |
dc.date.available | 2022-06-30T11:45:49Z | |
dc.date.issued | 2022-06-04 | |
dc.identifier.citation | Bartolomeu, A.R... [et al.]. Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p. Int. J. Mol. Sci. 2022, 23, 6292. [https://doi.org/10.3390/ijms23116292] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/75746 | |
dc.description.abstract | Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative, antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds. Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the earlystage of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a 1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF- , and downregulated the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated the genes involved in the negative regulation of proliferation and inflammation, and the positive regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF) development. Our findings suggest that a combination of coffee compounds reduces early-stage colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of coffee intake to prevent CRC. | es_ES |
dc.description.sponsorship | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 001 | es_ES |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2017/26217-7 2016/12015-0 2016/14420-0 | es_ES |
dc.description.sponsorship | Junta de Andalucia RH-0139-2020 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Caffeine | es_ES |
dc.subject | Chlorogenic acid | es_ES |
dc.subject | Chemically induced colon carcinogenesis | es_ES |
dc.subject | miRNA expression | es_ES |
dc.subject | Mice | es_ES |
dc.title | Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.3390/ijms23116292 | |
dc.type.hasVersion | VoR | es_ES |