Cytotoxicity and Wound Closure Evaluation in Skin Cell Lines after Treatment with Common Antiseptics for Clinical Use
Metadatos
Mostrar el registro completo del ítemAutor
Ortega Llamas, Laura; Quiñones Vico, María Isabel; García Valdivia, Marta; Fernández González, Ana; Ubago Rodríguez, Ana Dolores; Sanabria de la Torre, Raquel; Arias Santiago, Salvador AntonioEditorial
MDPI
Materia
Antiseptics Cell migration Cytotoxicity Fibroblasts Keratinocytes Wound healing Wound regeneration
Fecha
2022-04-20Referencia bibliográfica
Ortega-Llamas, L... [et al.]. Cytotoxicity and Wound Closure Evaluation in Skin Cell Lines after Treatment with Common Antiseptics for Clinical Use. Cells 2022, 11, 1395. [https://doi.org/10.3390/cells11091395]
Patrocinador
Spanish Government; Research, Development and Innovation in Biomedicine and Health Sciences in Andalusia PIGE-0242-2019; Instituto de Salud Carlos III PI17/02083Resumen
In recent years, new therapies, such as skin cell lines injections, have emerged to promote
re-epithelialization of damaged areas such as chronic ulcers or to treat patients with severe burns.
Antiseptics are commonly used during wound clinical management to avoid serious infections, but
they may delay the healing process due to their apparent cytotoxicity to skin cells. The cytotoxicity
of ethanol, chlorhexidine digluconate, sodium hypochlorite, povidone iodine and polyhexanide
was evaluated in this in vitro study on human fibroblasts and keratinocytes. Treatments were
applied to each cell type culture every 48 h for 14 days. To determine the cytotoxic of antiseptics,
cell viability (Live/Dead®) and cell proliferation (AlamarBlue™) assays were performed on cell
monolayers. Cell migration capacity was evaluated with a wound closure assay. Results showed
how chlorhexidine digluconate and ethanol significantly reduced the viability of keratinocytes and
inhibited cell migration. Povidone iodine followed by chlorhexidine digluconate significantly reduced
fibroblast cell viability. Povidone iodine also inhibited cell migration. Sodium hypochlorite was the
least detrimental to both cell types. If epithelial integrity is affected, the wound healing process may
be altered, so the information gathered in this study may be useful in selecting the least aggressive
antiseptic after treatment with new emerging therapies.