Cytotoxicity and Wound Closure Evaluation in Skin Cell Lines after Treatment with Common Antiseptics for Clinical Use Ortega Llamas, Laura Quiñones Vico, María Isabel García Valdivia, Marta Fernández González, Ana Ubago Rodríguez, Ana Dolores Sanabria de la Torre, Raquel Arias Santiago, Salvador Antonio Antiseptics Cell migration Cytotoxicity Fibroblasts Keratinocytes Wound healing Wound regeneration The work of Maria I. Quinones Vico is supported by a predoctoral fellowship (BOE 22/10/2019) from the Ministry of Science, Innovation and Universities of Spain. This study is part of her doctoral research in the Biomedicine's program of University of Granada. This research has received competitive funding in the call for grants for the financing of Research, Development and Innovation in Biomedicine and Health Sciences in Andalusia, for the year 2019 (PIGE-0242-2019) and from the Carlos III Health Institute (PI17/02083). In recent years, new therapies, such as skin cell lines injections, have emerged to promote re-epithelialization of damaged areas such as chronic ulcers or to treat patients with severe burns. Antiseptics are commonly used during wound clinical management to avoid serious infections, but they may delay the healing process due to their apparent cytotoxicity to skin cells. The cytotoxicity of ethanol, chlorhexidine digluconate, sodium hypochlorite, povidone iodine and polyhexanide was evaluated in this in vitro study on human fibroblasts and keratinocytes. Treatments were applied to each cell type culture every 48 h for 14 days. To determine the cytotoxic of antiseptics, cell viability (Live/Dead®) and cell proliferation (AlamarBlue™) assays were performed on cell monolayers. Cell migration capacity was evaluated with a wound closure assay. Results showed how chlorhexidine digluconate and ethanol significantly reduced the viability of keratinocytes and inhibited cell migration. Povidone iodine followed by chlorhexidine digluconate significantly reduced fibroblast cell viability. Povidone iodine also inhibited cell migration. Sodium hypochlorite was the least detrimental to both cell types. If epithelial integrity is affected, the wound healing process may be altered, so the information gathered in this study may be useful in selecting the least aggressive antiseptic after treatment with new emerging therapies. 2022-06-14T08:40:49Z 2022-06-14T08:40:49Z 2022-04-20 info:eu-repo/semantics/article Ortega-Llamas, L... [et al.]. Cytotoxicity and Wound Closure Evaluation in Skin Cell Lines after Treatment with Common Antiseptics for Clinical Use. Cells 2022, 11, 1395. [https://doi.org/10.3390/cells11091395] http://hdl.handle.net/10481/75475 10.3390/cells11091395 eng http://creativecommons.org/licenses/by/3.0/es/ http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Atribución 3.0 España MDPI