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dc.contributor.authorOliveira Amadeu, Simone
dc.contributor.authorGarcía Chaves, María Ángel
dc.identifier.citationPublished version: Amadeu, S... [et al.] (2022). Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis. British Journal of Nutrition, 1-11. doi:[10.1017/S0007114522001350]es_ES
dc.descriptionSarmiento-Machado L.M and Ariane Rocha Bartolomeu A.R received fellowships from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)- Finance code 001 and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-# 2017/26217-7, respectively.es_ES
dc.description.abstractColorectal cancer is the third most diagnosed cancer worldwide and linked to dietary/lifestyle factors. Arthrospira (Spirulina) platensis (AP) contains bioactive compounds with beneficial effects in vivo/in vitro. We evaluated the effects of AP feeding against 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male Sprague Dawley rats were given subcutaneous injections of DMH (4 × 40 mg/kg body weight) (G1–G3) or vehicle (G4–G5) twice a week (weeks 3–4). During weeks 1–4, animals were fed a diet containing 1 % (G2) or 2 % (G3–G4) AP powder (w/w). After this period, all groups received a balanced diet until week 12. Some animals were euthanised after the last DMH injection (week 4) for histological, immunohistochemical (Ki-67, γ-H2AX and caspase-3) and molecular analyses (real time-PCR for 91 genes), while other animals were euthanised at week 12 for preneoplastic aberrant crypt foci (ACF) analysis. Both AP treatments (G2–G3) significantly decreased the DMH-induced increase in γ-H2AX (DNA damage) and caspase 3 (DNA damage-induced cell death) in colonic crypts at week 4. In addition, Cyp2e1 (Drug metabolism), Notch1, Notch2 and Jag1 genes (Notch pathway) and Atm, Wee1, Chek2, Mgmt, Ogg1 and Xrcc6 genes (DNA repair) were also down-regulated by 2 % AP feeding (G3) at week 4. A significant reduction in ACF development was observed in both AP-treated groups (G2–G3) at week 12. In conclusion, findings indicate that AP feeding reduced acute colonic damage after DMH, resulting in fewer preneoplastic lesions. Our study provided mechanistic insights on dietary AP-preventive effects against early colon carcinogenesis.es_ES
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 001es_ES
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) 2017/26217-7es_ES
dc.publisherCambridge University Presses_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectArthrospira (Spirulina) platensises_ES
dc.subjectTumour initiationes_ES
dc.subjectNotch and DNA repair geneses_ES
dc.subjectColonic preneoplastic lesionses_ES
dc.subjectColon cancer preventiones_ES
dc.titleArthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesises_ES

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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España