dc.contributor.author | Garzarella, E. U. | |
dc.contributor.author | Navajas Porras, Beatriz | |
dc.contributor.author | Pérez Burillo, Sergio | |
dc.contributor.author | Hinojosa Nogueira, Daniel José | |
dc.contributor.author | Pastoriza de la Cueva, Silvia | |
dc.contributor.author | Rufián Henares, José Ángel | |
dc.contributor.author | Daglia, Maria | |
dc.date.accessioned | 2022-03-29T11:00:59Z | |
dc.date.available | 2022-03-29T11:00:59Z | |
dc.date.issued | 2022-03-03 | |
dc.identifier.citation | E.U. Garzarella et al. Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota. Biomedicine & Pharmacotherapy 148 (2022) 112759 [https://doi.org/10.1016/j.biopha.2022.112759] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/73899 | |
dc.description | CRediT authorship contribution statement
Emanuele Ugo Garzarella: Methodology, Data analysis, Data
curation, Software; Beatriz Navajas-Porras: Research design, Methodology,
Data analysis, Software; Sergio P´erez-Burillo: Writing
manuscript, Research design, Methodology, Data analysis; Hammad
Ullah: Writing manuscript; Cristina Esposito: Methodology, Data
analysis; Cristina Santarcangelo: Methodology, Data analysis; Daniel
Hinojosa-Nogueira: Research design, Data analysis; Silvia Pastoriza:
Research design; Vincenzo Zaccaria: Research design; Jianbo Xiao: Writing manuscript; Jos´e Angel Rufian-Henares: Writing manuscript,
Research design, Obtained funding; Maria Daglia: Conceptualization,
Writing manuscript. | es_ES |
dc.description | Author Contributions
S.P.B., H.U., J.A.R.H., J.X., and M.D. wrote or contributed to the
writing of the manuscript. S.P.B., J.A.R.H., B.N.P., D.H.N., V.Z., and S.P.
participated in research design. S.P.B., E.U.G., C.E., C.S., B.N.P., D.H.N.
conducted experiments and performed data analysis. J.A.R.H. obtained
funding for the study. All authors have read and agreed to the published
version of the manuscript. | es_ES |
dc.description.abstract | Introduction: A large body of evidence suggests that propolis exerts antioxidant, anti-inflammatory, and antimicrobial
activities, mostly ascribed to its polyphenol content. Growing evidence suggests that propolis could
modulate gut microbiota exerting a positive impact on several pathological conditions. The aim of this study was
to determine the in vitro impact of a poplar-type propolis extract with a standardized polyphenol content, on the
composition and functionality of gut microbiota obtained from fecal material of five different donors (healthy
adults, and healthy, obese, celiac, and food allergic children).
Methods: The standardized polyphenol mixture was submitted to a simulated in vitro digestion-fermentation
process, designed to mimic natural digestion in the human oral, gastric, and intestinal chambers. The antioxidant
profile of propolis before and after the digestion-fermentation process was determined. 16 S rRNA amplicon
next-generation sequencing (NGS) was used to test the effects on the gut microbiota of propolis extract. The
profile of the short-chain fatty acids (SCFA) produced by the microbiota was also investigated through a chromatographic
method coupled with UV detection.
Results: In vitro digestion and fermentation induced a decrease in the antioxidant profile of propolis (i.e., decrease
of total polyphenol content, antiradical and reducing activities). Propolis fermentation exhibited a modulatory
effect on gut microbiota composition and functionality of healthy and diseased subjects increasing the concentration
of SCFA.
Conclusions: Overall, these data suggest that propolis might contribute to gut health and could be a candidate for
further studies in view of its use as a prebiotic ingredient. | es_ES |
dc.description.sponsorship | Plan Propio de Investigación y
Transferencia of the University of Granada, Spain under the program
“Intensificación de la Investigación, modalidad B | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Poplar-type propolis | es_ES |
dc.subject | Multi dynamic extraction | es_ES |
dc.subject | In vitro digestion | es_ES |
dc.subject | In vitro fermentation | es_ES |
dc.subject | Antioxidant capacity | es_ES |
dc.subject | Gut microbiota | es_ES |
dc.subject | Short-chain fatty acids | es_ES |
dc.title | Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1016/j.biopha.2022.112759 | |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |