Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies
Metadatos
Afficher la notice complèteMateria
Cell culture Cell isolation Cancer Cell differentiation
Date
2020-12-18Referencia bibliográfica
Picon-Ruiz, M., Marchal, J. A., Slingerland, J. M. Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies. STAR Protocols, Volume 1, Issue 3, 2020, 100197
Patrocinador
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 845104Résumé
Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020).