Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies Picón Ruiz, Manuel Marchal Corrales, Juan Antonio Slingerland, Joyce Marie Cell culture Cell isolation Cancer Cell differentiation Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the in vitro study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020). 2022-03-24T07:58:28Z 2022-03-24T07:58:28Z 2020-12-18 info:eu-repo/semantics/article Picon-Ruiz, M., Marchal, J. A., Slingerland, J. M. Obtaining Human Breast Adipose Cells for Breast Cancer Cell Co-culture Studies. STAR Protocols, Volume 1, Issue 3, 2020, 100197 http://hdl.handle.net/10481/73670 10.1016/j.xpro.2020.100197 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España