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Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
dc.contributor.author | Fueyo González, Francisco | |
dc.contributor.author | Espinar Barranco, Laura | |
dc.contributor.author | Crovetto González, Luis | |
dc.contributor.author | Paredes Martínez, José Manuel | |
dc.contributor.author | Orte Gutiérrez, Ángel | |
dc.contributor.author | González Vera, Juan Antonio | |
dc.date.accessioned | 2022-02-04T11:35:42Z | |
dc.date.available | 2022-02-04T11:35:42Z | |
dc.date.issued | 2022-01-15 | |
dc.identifier.citation | ACS Sens. 2022, 7, 1, 322–330. [https://doi.org/10.1021/acssensors.1c02439] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/72669 | |
dc.description | This work was supported by grants CTQ2017-85658-R, BFU2015-67284-R, and PID2019-104366RB-C22 funded by MCIN/AEI/10.13039/501100011033/FEDER "Una manera de hacer Europa"; grant PID2020-114256RB-I00 funded by MCIN/AEI/10.13039/501100011033; grant A-FQM-386-UGR20 funded by FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades, and the CSIC grant 201580E073. Funding for open access charge: Universidad de Granada/CBUA. | es_ES |
dc.description.abstract | The small molecule 8-methoxy-2-oxo-1,2,4,5- tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves as a reactive non-fluorescent Michael acceptor, which after reaction with thiols becomes fluorescent, and an efficient Eu3+ antenna, after self-assembling with this cation in water. This behavior makes 2b a highly selective GSH biosensor, which has demonstrated high potential for studies in murine and human cells of the immune system (CD4+ T, CD8+ T, and B cells) using flow cytometry. GSH can be monitored by the fluorescence of the product of addition to 2b (445 nm) or by the luminescence of Eu3+ (592 nm). 2b was able to capture baseline differences in GSH intracellular levels among murine and human CD4+ T, CD8+ T, and B cells. We also successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of CD4+ naiv̈ e T cells into regulatory T cells (TREG). | es_ES |
dc.description.sponsorship | MCIN/AEI/FEDER "Una manera de hacer Europa" CTQ2017-85658-R BFU2015-67284-R PID2019-104366RB-C22 | es_ES |
dc.description.sponsorship | MCIN/AEI PID2020-114256RB-I00 | es_ES |
dc.description.sponsorship | FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades A-FQM-386-UGR20 | es_ES |
dc.description.sponsorship | CSIC grant 201580E073 | es_ES |
dc.description.sponsorship | Universidad de Granada/CBUA | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | Glutathione | es_ES |
dc.subject | Luminescent sensor | es_ES |
dc.subject | Self-assembled antenna | es_ES |
dc.subject | Lanthanide | es_ES |
dc.subject | Time-resolved luminescence | es_ES |
dc.subject | Flow cytometry | es_ES |
dc.subject | T cells | es_ES |
dc.subject | TREG | es_ES |
dc.title | Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1021/acssensors.1c02439 | |
dc.type.hasVersion | VoR | es_ES |