Self-Assembled Lanthanide Antenna Glutathione Sensor for the Study of Immune Cells
Metadata
Show full item recordAuthor
Fueyo González, Francisco; Espinar Barranco, Laura; Crovetto González, Luis; Paredes Martínez, José Manuel; Orte Gutiérrez, Ángel; González Vera, Juan AntonioEditorial
American Chemical Society
Materia
Glutathione Luminescent sensor Self-assembled antenna Lanthanide Time-resolved luminescence Flow cytometry T cells TREG
Date
2022-01-15Referencia bibliográfica
ACS Sens. 2022, 7, 1, 322–330. [https://doi.org/10.1021/acssensors.1c02439]
Sponsorship
MCIN/AEI/FEDER "Una manera de hacer Europa" CTQ2017-85658-R BFU2015-67284-R PID2019-104366RB-C22; MCIN/AEI PID2020-114256RB-I00; FEDER/Junta de Andalucia-Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades A-FQM-386-UGR20; CSIC grant 201580E073; Universidad de Granada/CBUAAbstract
The small molecule 8-methoxy-2-oxo-1,2,4,5-
tetrahydrocyclopenta[de]quinoline-3-carboxylic acid (2b) behaves
as a reactive non-fluorescent Michael acceptor, which after reaction
with thiols becomes fluorescent, and an efficient Eu3+ antenna,
after self-assembling with this cation in water. This behavior makes
2b a highly selective GSH biosensor, which has demonstrated high
potential for studies in murine and human cells of the immune
system (CD4+ T, CD8+ T, and B cells) using flow cytometry. GSH
can be monitored by the fluorescence of the product of addition to
2b (445 nm) or by the luminescence of Eu3+ (592 nm). 2b was
able to capture baseline differences in GSH intracellular levels
among murine and human CD4+ T, CD8+ T, and B cells. We also
successfully used 2b to monitor intracellular changes in GSH associated with the metabolic variations governing the induction of
CD4+ naiv̈ e T cells into regulatory T cells (TREG).