Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials.
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MDPI
Materia
Biofuel Ecodiesel Biodiésel Commercial CALB lipase Ordered mesoporous materials (PMO) Amorphous siliceous material MS3030
Date
2021-11-09Referencia bibliográfica
Luna, C.; Gascón-Pérez, V.; López-Tenllado, F.J.; Bautista, F.M.; Verdugo-Escamilla, C.; Aguado-Deblas, L.; Calero, J.; Romero, A.A.; Luna, D.; Estévez, R. Enzymatic Production of Ecodiesel by Using a Commercial Lipase CALB, Immobilized by Physical Adsorption on Mesoporous Organosilica Materials. Catalysts 2021, 11, 1350. [https://doi.org/10.3390/catal11111350]
Sponsorship
Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía; MICIIN PID2019-104953RB-100; MINECO-ENE2016-81013-R; UCO-FEDER 1264113-R, GOIPD/2015/287, P18-RT-4822; European Commission; European Regional Development Fund; Agencia Estatal de InvestigaciónAbstract
The synthesis of two biocatalysts based on a commercial Candida antarctica lipase B, CALB enzyme (E), physically immobilized on two silica supports, was carried out. The first support was a periodic mesoporous organosilica (PMO) and the second one was a commercial silica modified with octyl groups (octyl-MS3030). The maximum enzyme load was 122 mg enzyme/g support on PMO and 288 mg enzyme/g support on octyl-MS3030. In addition, the biocatalytic efficiency was corroborated by two reaction tests based on the hydrolysis of p-nitrophenylacetate (p-NPA) and tributyrin (TB). The transesterification of sunflower oil with ethanol was carried out over the bio-catalysts synthesized at the following reaction conditions: 6 mL sunflower oil, 1.75 mL EtOH, 30 °C, 25 μL NaOH 10 N and 300 rpm, attaining conversion values over 80% after 3 h of reaction time. According to the results obtained, we can confirm that these biocatalytic systems are viable candi-dates to develop, optimize and improve a new methodology to achieve the integration of glycerol in different monoacylglycerol molecules together with fatty acid ethyl esters (FAEE) molecules to obtain Ecodiesel.