Enhanced hydrogenation catalyst synthesized by Desulfovibrio desulfuricans exposed to a radio frequency magnetic field
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Microb. Biotechnol. (2021) 00( 00), 1– 18. [https://doi.org/10.1111/1751-7915.13878]
SponsorshipUK Research & Innovation (UKRI) Engineering & Physical Sciences Research Council (EPSRC) EP/I007806/1 EP/D05768X/1; UK Research & Innovation (UKRI) Biotechnology and Biological Sciences Research Council (BBSRC) BB/C516128/1; UK Research & Innovation (UKRI); Natural Environment Research Council (NERC) NE/L014076/1; Royal Society of London European Commission; Spanish Government Sistema Nacional de Garantia Juvenil grant PEJ-2014-P-00391
Desulfovibrio desulfuricans reduces Pd(II) to Pd(0)-nanoparticles (Pd-NPs) which are catalytically active in 2-pentyne hydrogenation. To make Pd-NPs, resting cells are challenged with Pd(II) ions (uptake), followed by addition of electron donor to promote bioreduction of cell-bound Pd(II) to Pd(0) (bio-Pd). Application of radiofrequency (RF) radiation to prepared 5 wt% bio-Pd catalyst (60 W power, 60 min) increased the hydrogenation rate by 70% with no adverse impact on selectivity to cis-2-pentene. Such treatment of a 5 wt% Pd/carbon commercial catalyst did not affect the conversion rate but reduced the selectivity. Lower-dose RF radiation (2-8 W power, 20 min) was applied to the bacteria at various stages before and during synthesis of the bio-scaffolded Pd-NPs. The reaction rate (mu mol 2-pentyne converted s(-1)) was increased by similar to threefold by treatment during bacterial catalyst synthesis. Application of RF radiation (2 or 4 W power) to resting cells prior to Pd(II) exposure affected the catalyst made subsequently, increasing the reaction rate by 50% as compared to untreated cells, while nearly doubling selectivity for cis 2-pentene. The results are discussed with respect to published and related work which shows altered dispersion of the Pd-NPs made following or during RF exposure.