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Monitoring of cyanotoxins in water from hypersaline microalgae colonies by ultra high performance liquid chromatography with diode array and tandem mass spectrometry detection following salting-out liquid-liquid extraction
dc.contributor.author | Hemmati, Maryam | |
dc.contributor.author | Tejada-Casado, Carmen | |
dc.contributor.author | Lara Vargas, Francisco Jesús | |
dc.contributor.author | García Campaña, Ana María | |
dc.contributor.author | Rajabi, Maryam | |
dc.contributor.author | del Olmo-Iruela, Monsalud | |
dc.date.accessioned | 2021-04-14T12:26:44Z | |
dc.date.available | 2021-04-14T12:26:44Z | |
dc.date.issued | 2019-07-29 | |
dc.identifier.citation | Hemmati, M., Tejada-Casado, C., Lara, F. J., García-Campaña, A. M., Rajabi, M., & del Olmo-Iruela, M. (2019). Monitoring of cyanotoxins in water from hypersaline microalgae colonies by ultra high performance liquid chromatography with diode array and tandem mass spectrometry detection following salting-out liquid-liquid extraction. Journal of Chromatography A, 1608, 460409. | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/67955 | |
dc.description.abstract | In this study two different analytical approaches have been developed to determine the presence of sev-eral cyanotoxins in saline water samples from a continental salt marsh. A salting-out assisted liquid-liquidextraction (SALLE) has been used in combination with ultra-high performance liquid chromatography-tandem mass spectrometry and UV-diode array detection (UHPLC-MS/MS and UHPLC-DAD). The targetanalytes are eight microcystins named MC-RR, MC-YR, MC-LR, MC-WR, MC-LA, MC-LY, MC-LW, MC-LFand nodularin (NOD), covering a wide range of polarities. The separation was achieved using a ZorbaxEclipse Plus RRHD C18 column (50 × 2.1 mm, 1.8 m) in less than 7.5 and 5.5 min for UV and MS/MSdetection, respectively. The mobile phase used consisted of water (solvent A) and acetonitrile (MeCN)(solvent B), both containing 0.01% of formic acid for DAD and 0.4% of formic acid for MS/MS detection,at a flow rate of 0.4 mL min−1. The temperature of the column was set at 25◦C and 20 L of samplewere injected. The main parameters affecting the SALLE procedure were studied and the following opti-mum values were obtained: neutral pH, 2 mL of acetonitrile as extraction solvent and 1.2 g of ammoniumsulfate as salting-out agent for 4 mL of water sample. The validation protocols for both methods wereaccomplished with real water samples obtaining LODs ranging from 1.0 to 3.4 g L−1and 0.02 to 0.11 gL−1for DAD and MS/MS respectively. Although the SALLE-UHPLC-DAD methodology is easier and cheaperthan UHPLC-MS/MS significantly better detection limits were achieved with tandem mass spectrometryas well as allowing for unambiguous identification. Extraction recoveries were higher than 77.0% (exceptfor MC-RR and NOD which were 53.2% and 54.3, respectively) with satisfactory inter-day and intra-dayprecisions (RSD below 13.3%). | es_ES |
dc.description.sponsorship | Departamento de Química Analítica, Grupo FQM 302 | es_ES |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (ProjectRef.: RTI2018-097043-B-I00) | es_ES |
dc.description.sponsorship | Ministry of Science, Research, and Technology of Iran | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | ELSEVIER | es_ES |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | Cyanotoxins | es_ES |
dc.subject | Diode array detection | es_ES |
dc.subject | Salting-out assisted liquid-liquid extraction | es_ES |
dc.subject | Saline waters | es_ES |
dc.subject | Tandem mass spectrometry | es_ES |
dc.subject | Ultra-high performance liquid chromatography | es_ES |
dc.title | Monitoring of cyanotoxins in water from hypersaline microalgae colonies by ultra high performance liquid chromatography with diode array and tandem mass spectrometry detection following salting-out liquid-liquid extraction | es_ES |
dc.type | journal article | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | https://doi.org/10.1016/j.chroma.2019.460409 | |
dc.type.hasVersion | SMUR | es_ES |