An effective polymeric nanocarrier that allows for active targeting and selective drug delivery in cell coculture systems
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AuthorCano Cortes, María Victoria; Altea Manzano, Patricia; Laz Ruiz, José Antonio; Unciti Broceta, Juan Diego; López Delgado, Francisco Javier; Espejo Román, José Manuel; Díaz Mochón, Juan José; Sánchez Martin, Rosario M.
Royal Society Chemistry
Cano-Cortes, M. V., Altea-Manzano, P., Laz-Ruiz, J. A., Unciti-Broceta, J. D., Lopez-Delgado, F. J., Espejo-Roman, J. M., ... & Sanchez-Martin, R. M. (2021). An effective polymeric nanocarrier that allows for active targeting and selective drug delivery in cell coculture systems. Nanoscale, 13(6), 3500-3511. [DOI: 10.1039/d0nr07145e]
SponsorshipHealth Institute Carlos III (ISCIII) DTS18/00121; Junta de Andalucia-FEDER, Ministry of Economy, Knowledge, Companies and University (University of Granada) B-FQM-475-UGR18 PT18-TP-1490 AT17_6096- OTRI; Spanish State Research Agency RED2018-102469-T; Fundación Benéfica Anticáncer San Francisco Javier y Santa Cándida; Spanish Ministry of Economy and Competitiveness PTQ-16- 08597; Spanish Government FPU 16/02061
In this manuscript, we report the development of a versatile, robust, and stable targeting nanocarrier for active delivery. This nanocarrier is based on bifunctionalized polymeric nanoparticles conjugated to a monoclonal antibody that allows for active targeting of either (i) a fluorophore for tracking or (ii) a drug for monitoring specific cell responses. This nanodevice can efficiently discriminate between cells in coculture based on the expression levels of cell surface receptors. As a proof of concept, we have demonstrated efficient delivery using a broadly established cell surface receptor as the target, the epidermal growth factor receptor (EGFR), which is overexpressed in several types of cancers. Additionally, a second validation of this nanodevice was successfully carried out using another cell surface receptor as the target, the cluster of differentiation 147 (CD147). Our results suggest that this versatile nanocarrier can be expanded to other cell receptors and bioactive cargoes, offering remarkable discrimination efficiency between cells with different expression levels of a specific marker. This work supports the ability of nanoplatforms to boost and improve the progress towards personalized medicine.