Methotrexate Gold Nanocarriers: Loading and Release Study: Its Activity in Colon and Lung Cancer Cells
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AuteurÁlvarez González, Beatriz; Rozalen, Marisa; Fernández Perales, María; Álvarez, Miguel A.; Sánchez Polo, Manuel
Au nanoparticlesNanocarriersMethotrexateAnticancer drugChemotherapeuticControlled release
Álvarez-González, B., Rozalen, M., Fernández-Perales, M., Álvarez, M. A., & Sánchez-Polo, M. (2020). Methotrexate Gold Nanocarriers: Loading and Release Study: Its Activity in Colon and Lung Cancer Cells. Molecules, 25(24), 6049. [doi:10.3390/molecules25246049]
PatrocinadorSpanish Government; Junta de Andalucía P18-RT-4193
In the present study, the synthesis of gold nanoparticles (AuNPs) loaded with methotrexate (MTX) has been carried out in order to obtain controlled size and monodispersed nanocarriers of around 20 nm. The characterization study shows metallic AuNPs with MTX polydispersed on the surface. MTX is linked by the replacement of citrate by the MTX carboxyl group. The drug release profiles show faster MTX release when it is conjugated, which leads to the best control of plasma concentration. Moreover, the enhanced release observed at pH 5 could take advantage of the pH gradients that exist in tumor microenvironments to achieve high local drug concentrations. AuNP–MTX conjugates were tested by flow cytometry against lung (A-549) and colon (HTC-116) cancer cell lines. Results for A-549 showed a weaker dose–response e ect than for colon cancer ones. This could be related to the presence of folate receptors in line HTC-116 in comparison to line A-549, supporting the specific uptake of folate-conjugated AuNP–MTX by folate receptor positive tumor cells. Conjugates exhibited considerably higher cytotoxic e ects compared with the e ects of equal doses of free MTX. Annexin V-PI tests sustained the cell death mechanism of apoptosis, which is normally disabled in cancer cells.