<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/66598">
      <dc:title>Methotrexate Gold Nanocarriers: Loading and Release Study: Its Activity in Colon and Lung Cancer Cells</dc:title>
      <dc:creator>Álvarez González, Beatriz</dc:creator>
      <dc:creator>Rozalen, Marisa</dc:creator>
      <dc:creator>Fernández Perales, María</dc:creator>
      <dc:creator>Álvarez, Miguel A.</dc:creator>
      <dc:creator>Sánchez Polo, Manuel</dc:creator>
      <dc:subject>Au nanoparticles</dc:subject>
      <dc:subject>Nanocarriers</dc:subject>
      <dc:subject>Methotrexate</dc:subject>
      <dc:subject>Anticancer drug</dc:subject>
      <dc:subject>Chemotherapeutic</dc:subject>
      <dc:subject>Controlled release</dc:subject>
      <dc:description>In the present study, the synthesis of gold nanoparticles (AuNPs) loaded with methotrexate&#xd;
(MTX) has been carried out in order to obtain controlled size and monodispersed nanocarriers of&#xd;
around 20 nm. The characterization study shows metallic AuNPs with MTX polydispersed on&#xd;
the surface. MTX is linked by the replacement of citrate by the MTX carboxyl group. The drug&#xd;
release profiles show faster MTX release when it is conjugated, which leads to the best control of&#xd;
plasma concentration. Moreover, the enhanced release observed at pH 5 could take advantage of&#xd;
the pH gradients that exist in tumor microenvironments to achieve high local drug concentrations.&#xd;
AuNP–MTX conjugates were tested by flow cytometry against lung (A-549) and colon (HTC-116)&#xd;
cancer cell lines. Results for A-549 showed a weaker dose–response e ect than for colon cancer ones.&#xd;
This could be related to the presence of folate receptors in line HTC-116 in comparison to line A-549,&#xd;
supporting the specific uptake of folate-conjugated AuNP–MTX by folate receptor positive tumor&#xd;
cells. Conjugates exhibited considerably higher cytotoxic e ects compared with the e ects of equal&#xd;
doses of free MTX. Annexin V-PI tests sustained the cell death mechanism of apoptosis, which is&#xd;
normally disabled in cancer cells.</dc:description>
      <dc:date>2021-02-16T08:49:33Z</dc:date>
      <dc:date>2021-02-16T08:49:33Z</dc:date>
      <dc:date>2020-12-21</dc:date>
      <dc:type>info:eu-repo/semantics/article</dc:type>
      <dc:identifier>Álvarez-González, B., Rozalen, M., Fernández-Perales, M., Álvarez, M. A., &amp; Sánchez-Polo, M. (2020). Methotrexate Gold Nanocarriers: Loading and Release Study: Its Activity in Colon and Lung Cancer Cells. Molecules, 25(24), 6049. [doi:10.3390/molecules25246049]</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/66598</dc:identifier>
      <dc:identifier>10.3390/molecules25246049</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>Mdpi</dc:publisher>
   </ow:Publication>
</rdf:RDF>