Gemcitabine-Loaded Magnetically Responsive Poly("-caprolactone) Nanoparticles against Breast Cancer
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AuthorGarcía García, Gracia; Fernández Álvarez, Fátima; Cabeza Montilla, Laura; Delgado Mora, Ángel Vicente; Melguizo Alonso, Consolación; Prados Salazar, José Carlos; Arias Mediano, José Luis
Breast cancerCore/shellDrug loadingGemcitabineMagnetic drug deliveryMagnetitepH-responsive drug releasePoly("-caprolactone)Polymer-coated nanoparticle
García-García, G., Fernández-Álvarez, F., Cabeza, L., Delgado, Á. V., Melguizo, C., Prados, J. C., & Arias, J. L. (2020). Gemcitabine-Loaded Magnetically Responsive Poly (ε-caprolactone) Nanoparticles against Breast Cancer. Polymers, 12(12), 2790. [doi:10.3390/polym12122790]
SponsorshipFEDER, Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+i), Instituto de Salud Carlos III (FIS, Spain) PI19/01478
A reproducible and efficient interfacial polymer disposition method has been used to formulatemagnetite/poly("-caprolactone) (core/shell) nanoparticles (average size 125 nm, production performance 90%). To demonstrate that the iron oxide nuclei were satisfactorily embedded within the polymeric solid matrix, a complete analysis of these nanocomposites by, e.g., electron microscopy visualizations, energy dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, electrophoresis, and contact angle goniometry was conducted. The magnetic responsive behaviour of these nanoparticles was quantitatively characterized by the hysteresis cycle and qualitatively investigated by visualization of the colloid under exposure to a 0.4 T magnet. Gemcitabine entrapment into the polymeric shell reported adequate drug loading values ( 11%), and a biphasic and pH-responsive drug release profile ( four-fold faster Gemcitabine release at pH 5.0 compared to pH 7.4). Cytotoxicity studies in MCF-7 human breast cancer cells proved that the half maximal inhibitory concentration of Gem-loaded nanocomposites was two-fold less than that of the free drug. Therefore, these core/shell nanoparticles could have great possibilities as a magnetically targeted Gemcitabine delivery system for breast cancer treatment.