Gemcitabine-Loaded Magnetically Responsive Poly("-caprolactone) Nanoparticles against Breast Cancer García García, Gracia Fernández Álvarez, Fátima Cabeza Montilla, Laura Delgado Mora, Ángel Vicente Melguizo Alonso, Consolación Prados Salazar, José Carlos Arias Mediano, José Luis Breast cancer Core/shell Drug loading Gemcitabine Magnetic drug delivery Magnetite pH-responsive drug release Poly("-caprolactone) Polymer-coated nanoparticle A reproducible and efficient interfacial polymer disposition method has been used to formulatemagnetite/poly("-caprolactone) (core/shell) nanoparticles (average size 125 nm, production performance 90%). To demonstrate that the iron oxide nuclei were satisfactorily embedded within the polymeric solid matrix, a complete analysis of these nanocomposites by, e.g., electron microscopy visualizations, energy dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, electrophoresis, and contact angle goniometry was conducted. The magnetic responsive behaviour of these nanoparticles was quantitatively characterized by the hysteresis cycle and qualitatively investigated by visualization of the colloid under exposure to a 0.4 T magnet. Gemcitabine entrapment into the polymeric shell reported adequate drug loading values ( 11%), and a biphasic and pH-responsive drug release profile ( four-fold faster Gemcitabine release at pH 5.0 compared to pH 7.4). Cytotoxicity studies in MCF-7 human breast cancer cells proved that the half maximal inhibitory concentration of Gem-loaded nanocomposites was two-fold less than that of the free drug. Therefore, these core/shell nanoparticles could have great possibilities as a magnetically targeted Gemcitabine delivery system for breast cancer treatment. 2021-02-11T08:56:37Z 2021-02-11T08:56:37Z 2020-11-25 journal article García-García, G., Fernández-Álvarez, F., Cabeza, L., Delgado, Á. V., Melguizo, C., Prados, J. C., & Arias, J. L. (2020). Gemcitabine-Loaded Magnetically Responsive Poly (ε-caprolactone) Nanoparticles against Breast Cancer. Polymers, 12(12), 2790. [doi:10.3390/polym12122790] http://hdl.handle.net/10481/66457 10.3390/polym12122790 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Mdpi