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dc.contributor.authorChato Astrain, Jesús 
dc.contributor.authorChato Astrain, Isabel
dc.contributor.authorSánchez Porras, David 
dc.contributor.authorGarcía García, Óscar Darío
dc.contributor.authorBermejo Casares, Fabiola
dc.contributor.authorGarzón Bello, Ingrid Johanna 
dc.contributor.authorCarriel Araya, Víctor 
dc.contributor.authorCampos, Fernando
dc.contributor.authorAlaminos Mingorance, Miguel 
dc.date.accessioned2021-02-04T11:13:26Z
dc.date.available2021-02-04T11:13:26Z
dc.date.issued2020-11-23
dc.identifier.citationChato-Astrain, J., Chato-Astrain, I., Sánchez-Porras, D., García-García, Ó. D., Bermejo-Casares, F., Vairo, C., ... & Alaminos, M. (2020). Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering. Journal of Nanobiotechnology, 18(1), 1-13. [https://doi.org/10.1186/s12951-020-00732-0]es_ES
dc.identifier.urihttp://hdl.handle.net/10481/66287
dc.description.abstractBackground: Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti-Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results: Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. Conclusions: These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibioticloaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.es_ES
dc.description.sponsorshipNanoGSkin project of EuroNanoMed-III (ERA-NET Cofund scheme of the Horizon 2020 Research and Innovation Framework Programme), EUes_ES
dc.description.sponsorshipInstituto de Salud Carlos III AC17/00013es_ES
dc.description.sponsorshipCentro para el Desarrollo Tecnológico Industrial -CDTI 00108589es_ES
dc.description.sponsorshipSpanish Governmentes_ES
dc.description.sponsorshipJunta de Andalucía PE-0395-2019es_ES
dc.description.sponsorshipFundacion Benefica Anticancer San Francisco Javier y Santa Candida, Granada, Spaines_ES
dc.description.sponsorshipDepartment of Economic Development and Infrastructure of the Basque Government budget, through the HAZITEK business R + D support program ZE-2017/00014es_ES
dc.description.sponsorshipEuropean Union (EU) OTRI.35A-07es_ES
dc.language.isoenges_ES
dc.publisherBmces_ES
dc.rightsAtribución 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/*
dc.subjectTissue engineeringes_ES
dc.subjectFunctionalizationes_ES
dc.subjectDermal substitutees_ES
dc.subjectSevere burnses_ES
dc.subjectHuman skines_ES
dc.subjectNanostructured lipid carrierses_ES
dc.subjectColistimethatees_ES
dc.subjectAmikacines_ES
dc.titleGeneration of a novel human dermal substitute functionalized with antibiotic‑loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineeringes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1186/s12951-020-00732-0
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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Atribución 3.0 España
Except where otherwise noted, this item's license is described as Atribución 3.0 España