The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer
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AuthorPisano, Andrea; Griñan Lison, Carmen; Farace, Cristiano; Jiménez González, Gema; Peña Martín, Jesús; Marchal Corrales, Juan Antonio
miR-486-5pColorectal cancerMetastasisCancer stem cellsSerum and stool biomarkers
Pisano, A., Griñan-Lison, C., Farace, C., Fiorito, G., Fenu, G., Jiménez, G., ... & Madeddu, R. (2020). The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer. Cancers, 12(11), 3432. [doi:10.3390/cancers12113432]
SponsorshipInstituto de Salud Carlos III PIE16-00045 DTS19/00145; Junta de Andalucía; European Union (EU) SOMM17/6109/UGR (UCE-PP2017-3); Chair "Doctors Galera-Requena in cancer stem cell research" CMC-CTS963; Fondazione Banco di Sardegna
Colorectal cancer (CRC) is the third most frequent cancer worldwide and the second cause of cancer deaths. Increasing evidences supports the idea that the poor prognosis of patients is related to the presence of cancer stem cells (CSCs), a cell population able to drive cancer recurrence and metastasis. The deregulation of microRNAs (miRNAs) plays a role in the formation of CSC. We investigated the role of hsa-miR-486-5p (miR-486-5p) in CRC, CSCs, and metastasis, in order to reach a better understanding of the biomolecular and epigenetic mechanisms mir-486-5p-related. The expression of miR-486-5p was investigated in three di erent matrices from CRC patients and controls and in CSCs obtained from the CRC cell lines HCT-116, HT-29, and T-84. In the human study, miR-486-5p was up-regulated in serum and stool of CRC patients in comparison with healthy controls but down-regulated in tumor tissue when compared with normal mucosa. miR-486-5p was also down-regulated in the sera of metastatic patients. In vitro, miR-486-5p was down-regulated in CSC models and it induced an inhibitory e ect on stem factors and oncogenes in the main pathways of CSCs. Our results provide a step forward in understanding the role of mir-486-5p in CRC and CSC, and suggest that further studies are needed to investigate its diagnostic and prognostic power, possibly in combination with other biomarkers.