@misc{10481/65579,
year = {2020},
month = {11},
url = {http://hdl.handle.net/10481/65579},
abstract = {Colorectal cancer (CRC) is the third most frequent cancer worldwide and the second
cause of cancer deaths. Increasing evidences supports the idea that the poor prognosis of patients is
related to the presence of cancer stem cells (CSCs), a cell population able to drive cancer recurrence
and metastasis. The deregulation of microRNAs (miRNAs) plays a role in the formation of CSC.
We investigated the role of hsa-miR-486-5p (miR-486-5p) in CRC, CSCs, and metastasis, in order to
reach a better understanding of the biomolecular and epigenetic mechanisms mir-486-5p-related.
The expression of miR-486-5p was investigated in three di erent matrices from CRC patients and
controls and in CSCs obtained from the CRC cell lines HCT-116, HT-29, and T-84. In the human
study, miR-486-5p was up-regulated in serum and stool of CRC patients in comparison with healthy
controls but down-regulated in tumor tissue when compared with normal mucosa. miR-486-5p was
also down-regulated in the sera of metastatic patients. In vitro, miR-486-5p was down-regulated in
CSC models and it induced an inhibitory e ect on stem factors and oncogenes in the main pathways
of CSCs. Our results provide a step forward in understanding the role of mir-486-5p in CRC and
CSC, and suggest that further studies are needed to investigate its diagnostic and prognostic power,
possibly in combination with other biomarkers.},
organization = {Instituto de Salud Carlos III

PIE16-00045
DTS19/00145},
organization = {Junta de Andalucía},
organization = {European Union (EU)

SOMM17/6109/UGR (UCE-PP2017-3)},
organization = {Chair "Doctors Galera-Requena in cancer stem cell research" 	
CMC-CTS963},
organization = {Fondazione Banco di Sardegna},
publisher = {Mdpi},
keywords = {miR-486-5p},
keywords = {Colorectal cancer},
keywords = {Metastasis},
keywords = {Cancer stem cells},
keywords = {Serum and stool biomarkers},
title = {The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer},
doi = {10.3390/cancers12113432},
author = {Pisano, Andrea and Griñán Lisón, Carmen and Farace, Cristiano and Jiménez González, Gema and Peña Martín, Jesús and Marchal Corrales, Juan Antonio},
}