A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR
Metadatos
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Royal Society Chemistry
Materia
Maleic Acid Copolymer Modulation Bilayer Inserts Channel
Fecha
2020-10-07Referencia bibliográfica
Medina-Carmona, E., Varela, L., Hendry, A. C., Thompson, G. S., White, L. J., Boles, J. E., ... & Ortega-Roldan, J. L. (2020). A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chemical Communications, 56(78), 11665-11668.[DOI: 10.1039/d0cc03612a]
Patrocinador
Wellcome Trust 207743/Z/17/Z; Royal Society of London RGS\R1\191414; Public Health EnglandResumen
The activity of membrane proteins and compounds that interact
with the membrane is modulated by the surrounding lipid composition.
However, there are no simple methods that determine the
composition of these annular phospholipids in eukaryotic systems.
Herein, we describe a simple methodology that enables the identification
and quantification of the lipid composition around
membrane-associated compounds using SMA-nanodiscs and routine
1H–31P NMR.