A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR

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URI: http://hdl.handle.net/10481/64106
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Author
Medina Carmona, Encarnación
Editorial
Royal Society Chemistry
Materia
Maleic Acid Copolymer
 
Modulation
 
Bilayer
 
Inserts
 
Channel
 
Date
2020-10-07
Referencia bibliográfica
Medina-Carmona, E., Varela, L., Hendry, A. C., Thompson, G. S., White, L. J., Boles, J. E., ... & Ortega-Roldan, J. L. (2020). A quantitative assay to study the lipid selectivity of membrane-associated systems using solution NMR. Chemical Communications, 56(78), 11665-11668.[DOI: 10.1039/d0cc03612a]
Sponsorship
Wellcome Trust 207743/Z/17/Z; Royal Society of London RGS\R1\191414; Public Health England
Abstract
The activity of membrane proteins and compounds that interact with the membrane is modulated by the surrounding lipid composition. However, there are no simple methods that determine the composition of these annular phospholipids in eukaryotic systems. Herein, we describe a simple methodology that enables the identification and quantification of the lipid composition around membrane-associated compounds using SMA-nanodiscs and routine 1H–31P NMR.
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Except where otherwise noted, this item's license is described as Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License