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dc.contributor.authorWijn, Raphaël de
dc.contributor.authorGavira, José A.
dc.date.accessioned2020-04-23T11:50:34Z
dc.date.available2020-04-23T11:50:34Z
dc.date.issued2019
dc.identifier.citationde Wijn, R., Hennig, O., Roche, J., Engilberge, S., Rollet, K., Fernandez-Millan, P., ... & Girard, E. (2019). A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography. IUCrJ, 6(3).es_ES
dc.identifier.urihttp://hdl.handle.net/10481/61535
dc.description.abstractDetermining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine ‘nonmodel’ and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip conceptes_ES
dc.description.sponsorshipThe following funding is acknowledged: Agence Nationale de la Recherche (contract No. ANR-11-LABX- 0057_MITOCROSS to Claude Sauter, Bernard Lorber; contract No. ANR-10-LABX-0036_NETRN to Claude Sauter, Bernard Lorber; contract No. ANR-13-BS07-0007-01 to Eric Girard, Sylvain Engilberge); Ministère des Affaires Etrangères (contract No. PROCOPE Hubert Curien to Claude Sauter, Mario Mörl); Deutsche Forschungsgemeinschaft (contract No. Mo 634/10-1 to Mario Mörl, Heike Betat); Université de Strasbourg [grant No. Initiative d’excellence (IDEX) to Claude Sauter, Raphaël de Wijn]; Centre National de la Recherche Scientifique (grant No. MRCT- 2012_PTI_UPR9002 to Claude Sauter).es_ES
dc.language.isoenges_ES
dc.publisherInternational Union of Crystallographyes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectMacromolecules es_ES
dc.subjectCrystallization es_ES
dc.subjectCounter-diffusiones_ES
dc.subjectMicrofluidicses_ES
dc.subjectSeedinges_ES
dc.titleA simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallographyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.1107/S2052252519003622


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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España