A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
Metadata
Show full item recordEditorial
International Union of Crystallography
Materia
Macromolecules Crystallization Counter-diffusion Microfluidics Seeding
Date
2019Referencia bibliográfica
de Wijn, R., Hennig, O., Roche, J., Engilberge, S., Rollet, K., Fernandez-Millan, P., ... & Girard, E. (2019). A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography. IUCrJ, 6(3).
Sponsorship
The following funding is acknowledged: Agence Nationale de la Recherche (contract No. ANR-11-LABX- 0057_MITOCROSS to Claude Sauter, Bernard Lorber; contract No. ANR-10-LABX-0036_NETRN to Claude Sauter, Bernard Lorber; contract No. ANR-13-BS07-0007-01 to Eric Girard, Sylvain Engilberge); Ministère des Affaires Etrangères (contract No. PROCOPE Hubert Curien to Claude Sauter, Mario Mörl); Deutsche Forschungsgemeinschaft (contract No. Mo 634/10-1 to Mario Mörl, Heike Betat); Université de Strasbourg [grant No. Initiative d’excellence (IDEX) to Claude Sauter, Raphaël de Wijn]; Centre National de la Recherche Scientifique (grant No. MRCT- 2012_PTI_UPR9002 to Claude Sauter).Abstract
Determining optimal conditions for the production of well diffracting crystals is
a key step in every biocrystallography project. Here, a microfluidic device is
described that enables the production of crystals by counter-diffusion and their
direct on-chip analysis by serial crystallography at room temperature. Nine ‘nonmodel’
and diverse biomacromolecules, including seven soluble proteins, a
membrane protein and an RNA duplex, were crystallized and treated on-chip
with a variety of standard techniques including micro-seeding, crystal soaking
with ligands and crystal detection by fluorescence. Furthermore, the crystal
structures of four proteins and an RNA were determined based on serial data
collected on four synchrotron beamlines, demonstrating the general applicability
of this multipurpose chip concept