Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated in vitro with different NSAIDs. Expression of RUNX-2, COL-I, OSX, was reduced by treatment with all studied NSAIDs, OSC expression was reduced by all NSAIDs except for ketoprofen, naproxen, or piroxicam. Expression of BMP-7 was reduced by all NSAIDs; BMP-2 was reduced by all except for naproxen. In general, NSAID treatment increased the expression of TGF- 1, but not of its receptors (TGF -R1, TGF -R2, and TFG -R3), which was either unchanged or reduced by the treatment. These data confirm that NSAIDs can affect osteoblast physiology, suggesting their possible impact on bone.