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Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
dc.contributor.author | Álvarez Cubero, María Jesús | |
dc.contributor.author | Santiago, O. | |
dc.contributor.author | Martínez Labarga, Cristina | |
dc.contributor.author | Martínez-García, B. | |
dc.contributor.author | Marrero-Díaz, R. | |
dc.contributor.author | Rubio-Roldán, A. | |
dc.contributor.author | Pérez-Gutiérrez, A. M. | |
dc.contributor.author | Carmona Sáez, Pedro | |
dc.contributor.author | Lorente Acosta, José Antonio | |
dc.contributor.author | Martínez González, Luis Javier | |
dc.date.accessioned | 2019-08-09T07:40:23Z | |
dc.date.available | 2019-08-09T07:40:23Z | |
dc.date.issued | 2018-06-21 | |
dc.identifier.citation | Álvarez-Cubero, María Jesús; et. al. Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads. Scientific Reports (2018) 8:9436 [DOI:10.1038/s41598-018-27819-x] | es_ES |
dc.identifier.uri | http://hdl.handle.net/10481/56613 | |
dc.description.abstract | This study is a comparison of the efficiency of three technologies used for Y chromosome capture and the next-generation sequencing (NGS) technologies applied for determining its whole sequence. Our main findings disclose that streptavidin–biotin magnetic particle-based capture methodology offers better and a deeper sequence coverage for Y chromosome capture, compared to chromosome sorting and microdissection procedures. Moreover, this methodology is less time consuming and the most selective for capturing only Y chromosomal material, in contrast with other methodologies that result in considerable background material from other, non-targeted chromosomes. NGS results compared between two platforms, NextSeq 500 and SOLID 5500xl, produce the same coverage results. This is the first study to explore a methodological comparison of Y chromosome capture and genetic analysis. Our results indicate an improved strategy for Y chromosome research with applications in several scientific fields where this chromosome plays an important role, such as forensics, medical sciences, molecular anthropology and cancer sciences. | es_ES |
dc.description.sponsorship | Spanish Alfonso Martin Escudero Foundation for the financial support to one of the authors of the present work (MJ Alvarez –Cubero). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.rights | Atribución 3.0 España | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.title | Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.doi | 10.1038/s41598-018-27819-x |